辣椒泛素交联酶cDNA的分离及其在UV-B作用下的表达分析

Isolation of ubiquitin-conjugating enzyme cDNA from Capsicum annuum and analysis of its expression under UV-B stress

利用96孔板法从UV处理的辣椒叶片cDNA文库中分离获得了一个cDNA阳性克隆,其长度为678 bp,含有148个氨基酸的开放读码框,与马铃薯等其他植物的泛素交联酶基因有较高的同源性,命名为CaE21.荧光定量PCR分析表明CaE21基因在UV-B作用下转录表达水平降低;当UV-B照射停止后,其表达水平逐渐回升.推测该基因与辣椒适应UV-B照射胁迫有关.

One cDNA positive clone of Capsicum annuum was isolated from a cDNA library constructed from leaves of C. annuum challenged with UV by a method of PCR-based 96-hole screening. The positive clone shared a high similarity with other plants＇ E2, named CaE21. From the result of real-time PCR, the transcription of CaE21 decreased under the UV-B treatment, and the transcription of CaE21 increased to its normal level 24h after the UV-B radiation stopped. The result indicated that CaE21 might have a specifrc function in the adaptation of C. annuum to UV-B radiation.