香加皮不同提取部位体外抗肿瘤活性实验研究

Antitumor activity of extract from different parts of Periploca Sepium Bge.

[目的]筛选香加皮的抗肿瘤活性部位。[方法]用大孔吸附树脂柱层析法对香加皮的和60%乙醇回流提取物进行分段处理,利用四甲基偶氮唑盐(MTT)法测定各提取部位体外抗肿瘤活性。[结果]70%乙醇洗脱部位对体外肿瘤细胞表现出抑制活性,并在测定浓度范围内呈现出良好剂量依赖性。95%乙醇洗脱部位的抑制作用较弱。而水洗脱物和20%乙醇洗脱物则未呈现出抗肿瘤活性。在各提取部位中70%乙醇提取物活性最强。[结论]70%乙醇提取部位是香加皮主要的体外抗肿瘤活性部位。

[Objective] To screen the part of Periploca Sepium Bge.（PSBF） with antitumor activity. [Methods] The extract of Periploca Sepium Bge. with 60% ethanol was treated in the way of macroporous adsorptive resins column chromatography to afford different fractions. The antitumor activities of the different fractions in vitro were determined by MTT assay. [Results] The 70% ethanol elution（PSBF-C） showed strong inhibitory effect on growth of cancer cells with significant dosage-dependence. The 95% ethanol elution （PSBF-D） showed low antitumor activity in vitro. And the most sensitive tumor cell lines to PSBF-C and PSBF-D were human hepatocellular carcinoma SMMC-7721 cell line. Whereas the fraction of 20% ethanol/H2O elution showed no antitumor activity. [Conclusions] The extract of 70% ehanol elution was the principal antitumor fraction from Periploca Sepium Bge.