薄层扫描法测定清肺消炎丸中岩白菜素的含量

Content determination of arolisic acid B in Qingfei Xiaoyan Pills with Thin-layer chromatography scanning method

[目的]建立清肺消炎丸中岩白菜素的含量测定方法。[方法]采用甲醇超声,乙醚除杂质,乙酸乙酯提取,双波长反射锯齿扫描法测定清肺消炎丸中岩白菜素的含量(276365)。[结果]岩白菜素在0.538～3.228μg有良好的线性关系,r=0.998(n=6),平均回收率98.19%,峰面积(RSD)=1.39%。[结论]该方法简便、准确,专属性强,可作为控制清肺消炎丸质量的方法。

[Objective] To establish a method for determining arolisic acid B in Qingfei Xiaoyan Pills with thin-layer chromatography scanning （TLCS）. [Methods] The content of arolisic acid B in Qingfei Xiaoyan Pills was determined with TLCS method （276 365）. [Results] The concentration of 0.538-3.228 μg arolisic acid B was in a good linear relationship（r=0.998）. The average recovery was 98.19% and RSD was 1.39%. [Conclusions] The detection method was simple, easy, and can be used for content determination of arolisic acid B in Qingfei Xiaoyan Pills.