p63和p504S联合检测在前列腺肿瘤鉴别诊断中的应用

Application of p63 and p504S in Diagnosis of Prostate Cancer

目的探讨p63和p504S联合免疫组化染色在前列腺良、恶性病变鉴别诊断中的价值。方法采用免疫组化方法,观察p63、p504S在不同前列腺疾病中的表达情况。结果大多数良性前列腺增生及低度PIN的腺泡和导管周围可见连续的p63(+),少数呈间断表达,p504S染色皆呈(-);高度PIN p63染色呈不连续表达或(-),而增生的腺上皮,部分细胞p504S呈弱(+)或(+);非典型腺瘤性增生p63呈(+),p504S呈阴性反应;前列腺癌20例中19例p63染色呈(-),1例显示局灶性(+),但p504S均呈(+)。结论p63、p504S联合检测可极大地提高前列腺癌诊断的正确率。

Objective To evaluate the value of p63 and p504S immunostaining in diagnosis of benign and malignant lesions of the prostate. Methods Expressions of p63 and p504S in benign and malignant lesions of prostate were observed by.immunohistochemical method. Results Continuous positive expressions of p63 were found in the acini and ducts of most benign prostatic hyperplasia and low prostatic intraepithelial neoplasia （PIN） , and few cases showed interrupted expressions, p504S staining presented negative reaction; Interrupted expressions or negative reactions were found in high PIN cases and a part of hyperplastie glandular epithelia presented weekly positive or positive for p504S ; Atypical aolenomatous hyperplasia was positive for p63, but negative for p504S; p63 staining was negative in 19 cases of 20 prostatic carcinoma cases, 1 case presented focally positive reaction by p63, but negative by p504S. Conclusions Combined detection of p63 and p504S can be most helpful for diagnosis of prostatic carcinoma.