不良妊娠女性162例生殖道中解脲脲原体生物一群的分布状况研究

Study on Distribution of Biotype One of Ureaplasma Urealyticum in Genital Duct of 162 Pregnant Women with Unfavorable Pregnancy Outcome

目的 研究不良妊娠女性生殖道中解脲脲原体生物一群的感染或定植情况及其血清分型。方法 选择2006年4月。2007年3月间就诊的不良妊娠孕妇162例为研究组，同期入产房待产或引产的健康孕妇89例为对照组，采集其宫颈分泌物进行解脲脲原体（Uu）液体培养，初筛阳性的液体培养基用煮沸法提取DNA，用PCR法进行血清分型。结果 162例不良妊娠女性生殖道中Uu生物一群的阳性检出率为74．69％，健康孕妇阳性检出率为47．19％，差异有显著性意义（P〈0．05）。血清1型、14型及混合型在不良妊娠组中的分布高于健康孕妇组，差异有显著性意义（P〈0．05）；3型、6型在两组中分布差异无显著性意义（P〉0．05）。结论 Uu生物一群与不良妊娠的发生可能有关；1型、14型及混合型可能是致病菌，3型、6型可能正常被携带。

Objective To investigate the infection or colonization of semtypes of biotype one of Ureaplasma urealyticum in genital duct of pregnant women with unfavorable pregnancy outcome and the relationships. Methods 251 pregnant women in department of Obstetrics and Gynecology from April 2006 to March 2007 were colonized by Ureaplasma urealyticum as the sole pathogenic microorganism, Cervical swabs were taken from 89 clients of normal pregnancy and 162 clients of clinical services with unfavorable pregnancy outcome were cultured for 24 hours.Positive cultures were further genotypes by polymerase chain reaction （PCR）.Results In 162 pregnant women with unfavorable pregnancy outcome, the total positive rates of biotype one was 74.69% （P 〈 0.05）. There were significant different in the distribution pattern of serotypes of biotype one in the different cases. The serotype 1, serotype 14, mixed serotype were more frequentlypresent in pregnant women with unfavorable pregnancy outcome and the serotype 3 and serotype 6 was mere frequently present in the other ones. Conclusion There may be correlation between biotype one of Uu and unfavorable pregnancy outcome, moreover, the serotype 1, serotype 14, mixed serotype of Uu may be the pathogenic microorganisms.