β_2肾上腺素受体多态性与慢性阻塞性肺疾病的关系探讨

The Study of β_2-AR Polymorphisms and Chronic Obstructive Pulmonary Disease on Relations

目的探讨β2肾上腺素受体(β2-AR)编码区16位点基因多态性与慢性阻塞性肺病(COPD)的关系。方法通过测序确定95例受试者的基因突变类型(正常对照组48例,COPD组47例),并同时测定COPD患者的FEV1%.结果两组β2-AR16位均以精基酸(Arg)16 Arg纯合子基因型为主,而β2-AR16位Arg16甘氨酸(Gly)杂合子及Gly16Gly纯合子较少,两组间基因频率比较差异无显著性;β2-AR Gly16等位基因频率在两组间比较差异有显著性,其在COPD组的频率明显高于正常对照组。结论β2-AR16位基因多态性可能与COPD的发病无关,但β2-AR Gly16位等位基因可能与COPD有关。

Objective To investigate the relationship between the genetic polymorphisms of coding region of adrenoeeptor（β2-AR） in 16 position and chronic obstructive puhnonary disease（COPD）. Methods The sequencing technique was used to determine 16 locos of β2-AR genetic polymorphism in 47 unrelated patients with chronic obstructive puhnonary disease and 48 healthy controls. Puhnonary funetion tests were performed in allpartieipants. Results The most genotypes of β2-AR in 16 position in the two groups were Arg16Arg homozygote,while the 16Gly heternzygote and Gir16 homozygote were rare. There were no significant difference the frequencies of genotypes of β2-AR16 loci in the two groups. The β2-AR Gly16 allele frequency in the two groups were difference,it was obviously higher in the COPD groups than the health controls. Conclusion The genetic polymorphisms of β2-AR in 16 position was not the basle agent of COPD. The allele frequencies of β2-AR in 16 position possibly were related with the agent agent COPD.