霉菌TM-3的成球条件及其对染料的吸附脱色研究

Study on Pelletization Conditions of Mould TM-3 and the Bioabsorption and Decolourization for Dyestuffs

通过对霉菌TM-3培养条件的研究，考查了培养条件对霉菌菌丝球产量、菌球特征及菌球吸附脱色性能的影响。实验结果表明：该霉菌在以蔗糖为碳源，乙酸胺为氮源时，250mL三角瓶装液量为100～125mL，1％接种量（孢子悬液浓度10^4），pH6，34℃，150r／min摇床培养60-72h时，可形成直径在1．O～2．5mm范围内的菌丝球，生物量可达150～200g／L（重）。球体白色光滑均匀，具有一定的机械强度，对10mg/L的酸性大红和200mg／L的刚果红吸附脱色达80％以上。培养条件的改变对菌球的特征、产量影响较大，对菌球吸附性能的影响相对较小。

The culture conditions of mould TM--3 were studied for examining the culture conditions which make a difference to the yield of mould mycelium pellet , the character of mould mycelium pellet and the performance of bioadsorption and decolorization. The experimental results showed that this mould was found when take cane sugar as the carbon source, taken acetamide as nitrogen source , the bottled liquid amount was 100-125mL, 1% inoculum size（ sporule suspension concentration was 106 pcs/mL） was put into 250mL triangle bottle, and this mould was trained for 60-72h by shaking table which rotate speed was 150r/min, pH was 5, temperature was 34℃. Under the conditions this mould could be shaped mycelium pellet which diameter is 1.0-2. 5mm , and biomass could be 150-200g/L （wet weight）. The mycelium pellet is lubricity and uniformity , and it has definite mechanical strength; and the rate of absorption and decolorization for 10mg/L acid brilliant scarlet and 200mg/L Congo red was more than 80%. The change of the conditions had a greater influence on the yield of mould mycelium pellet and the character of mould mycelium pellet , but the influence on the performance of bioadsorption and decolorization was relatively small.