鲟鱼原肌球蛋白的提取、结晶与电镜观察

Purification Crysttalization and Electromicroscopic Observation of Tropomyosin of Sturgeon

本文对黑龙江省特产的软骨硬鳞鱼、鲟形目的重要代表动物鲟鱼的原肌球蛋白(Tropomyosin以下用TM表示)进行了提取、纯化和结晶,并对原肌球蛋白晶体做了光学显微镜和电子显微镜观察。在光镜下可见到原肌球蛋白或聚或散的针状结晶。在电镜下可看到鲟鱼原肌球蛋白晶体具有清晰的、明暗相间的原肌球蛋白所特有的特征性横纹周期,在横纹主周期另有若干横纹细节。

Tropomyosin (TM) was isolated and purified from the body muscle of sturgeon [Acipenser schrencki (Brandt)]. Tropomyosin of sturgeon was crystalized in the presence of 0.05M MgC1_2— 0.05M Tris—HCl (pH7) .The crystal of TM was characterized with its needle-shape and revealed a typical and distinctive striations with a period 40nm.Some details could be seen on it.