GS0202菌产人参皂苷-β-葡萄糖苷酶条件及其酶的反应条件

Production and characterization of ginsenoside-β-glucosidase from bacteria GS0202

为提高Arthrobacter chlorophenolicus GS0202菌所产人参皂苷-β-葡萄糖苷酶水解人参皂苷Rb1生成人参皂苷Rg3的能力,采用单因素研究方法,对菌体发酵条件,及其所产酶的酶反应条件进行了研究。结果表明,菌体发酵的最适培养基组成为胰蛋白胨10 g,酵母粉5 g,氯化钠10 g,水1 L;诱导物为人参总皂苷,其加入量为3.2 mg/mL,培养温度为28℃;酶反应的最适条件为25℃,pH 4,底物质量浓度为3 mg/mL。

Production of the ginsenoside-β-glucosidasebybacteria GS0202 was optimized. The medium composition is proteose peptone 10 g, yeast powder 5 g, NaCI 10 g. 3.2 mg/mL total saponins of Panax ginseng and water 1 L and culture temperature is 28 ℃. The opimum temperature for enzyme catalysis is 25℃, pH 4 and the concentration of substrate is 3 mg/mL.