瓜蒌皮提取物对大鼠动脉粥样硬化保护作用的实验研究

Experimental Studies on Protective Effect of EPT on Atherosclerosis in Mice

目的观察瓜萎皮提取物对高脂饮食致大鼠动脉粥样硬化的保护作用．方法健康Wistar大鼠，雌雄不限，随机分3组：正常对照组、高脂模型组、瓜蒌皮提取物药物组，连续喂饲、灌胃11周．实验结束后检测血脂水平。光镜下观察主动脉弓形态学变化，免疫组织化学染色法检测胸主动脉细胞间粘附分子-1（ICAM-1）的表达．结果瓜萎皮提取物给药组大鼠血清总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-c）和动脉粥样硬化指数（AI）明显低于高脂模型组，两者比较。差异非常显著（P〈0．01，P〈0．05）；光镜下模型组血管壁可见典型粥样硬化斑块，药物组血管壁结构变化轻微，接近正常组；免疫组织化学染色结果显示模型组ICAM-1呈高表达，正常组呈现基础量的表达，药物组明显弱于模型组．结论瓜蒌皮提取物可降低大鼠血清总胆固醇，下调ICAM-1的表达量，对实验性大鼠高脂血症所致动脉粥样硬化有明显的保护作用．

Objective To observe the protective effect of EPT on atherosclerosis in mice. Methods Healthy Wistar mice were divided into 3 groups randomly. Groups A, B and C were given with common forage, high fat forage, high fat forage with EPT respectively. After 11 weeks feeding, serum levels of total cholesterol（TC） triglyceride（ TG）, low-density lipoprotein cholesterol （ LDL-C ）, high-density lipoprotein cholesterol （ HDL-C ） were detected. To observe changes of aorta arch under the light microscope and to evaluate the expression of intercellular adhesion molecule-1 in breast aorta with immunohistochemistry technology. Results EPT decreased the serum levels of TC, LDL-c and AI obviously. The difference between model group and medicine group is remarkable（P 〈 0.01, P 〈 0.05 ）. The staining of immunohistochemistry showed high expression of ICAM-1 in group B and basic expression in group A. Drug group was fainter clearly than group B. Conclusion EPT has a good effect in preventing and treating AS from experimental hyperlipidemia in mice by decreasing serum levels of TC, LDL-c and the expression of ICAM-1.