Nucleostemin和Ki-67在垂体腺瘤组织中的表达及其临床意义

Expressions of nucleostemin and Ki-67 in pituitary adenomas and their cfinical significance

目的研究nucleostemin基因和Ki-67抗原在垂体腺瘤中的表达，分析nucleostemin基因在垂体腺瘤细胞增殖及肿瘤发生和发展中的作用。方法收集垂体腺瘤手术标本71例，采用半定量RT-PCR方法检测垂体腺瘤标本中nucleosteminmRNA的表达情况，采用免疫组化方法检测垂体腺瘤石蜡切片中Ki-67抗原的表达情况。同时收集相关临床资料。结果本组71例垂体腺瘤手术标本均有nucleosteminmRNA表达。非侵袭性垂体腺瘤和侵袭性垂体腺瘤中nucleosteminmRNA、Ki-67标记指数（Ki-67LI）表达差异有统计学意义（P〈0．05）。复发组与未复发组中Ki-67LI表达差异有统计学意义（P〈0．05）。Nucleostemin mRNA表达水平与Ki-67LI呈正相关关系（r=0．237。P〈0．05）。结论Nucleostemin基因在人垂体腺瘤的侵袭性行为中发挥重要作用，其表达增高和Ki-67表达水平升高之间存在正相关，二者可以作为判断垂体腺瘤侵袭性和预测复发的有效临床检测指标。

Objective To study the expressions of nucleostemin gene mRNA and Ki-67 antigen in pituitary adenomas and investigate the role of nucleostemin gene in the tumorigenesis and development of pituitary adenomas. Methods Seventy-one samples of pituitary adenomas were collected. Semi-quantitative reverse transcriptase-polymerase chain reaction （RT-PCR） was used to detect the expression of nucleostemin gene mRNA in samples. Immunohistochemistry technique was applied to examine Ki-67 antigen expression in the paraffin sections of samples. At the same time, coherent clinical data were collected. Results Nucleostemin gene mRNA was detectable in all samples of pituitary adenomas. Between invasive and noninvasive pituitary adenomas, the difference of nucleostemin expression was extremely significant （P〈0.01）, and Ki-67 labeling index （LI） was also significantly different （P〈0.05）. The difference of Ki-67LI between recurrent and non-recurrent groups was significant （P〈0.05）. There was a positive correlation between nucleostemin gene and Ki-67LI levels （r =0.237, P〈0.05）. Conclusions Nucleostemin gene plays an important roles in the invasion of human pituitary adenoma. Expression of nucleostemin gene is positively related to Ki-67 antigen expression, and both may be the valid clinical detection markers for assessing proliferation, invasion and recurrence of pituitary adenomas.