可变高氧环境下SD大鼠可形成ROP模型

Retinopathy of prematurity model can be developed in newborn SD rats in variable oxygen concentration

目的：可变氧环境下形成SD大鼠ROP模型，为动物实验研究提供ROP模型。方法：将新生24只SD乳鼠及母鼠放入密闭氧箱内饲养，箱内氧浓度为400，800mL/L各12h交替循环14d，再将大鼠放至正常氧环境下饲养；间接检眼镜观察高氧后SD鼠眼底变化；视网膜血管内皮细胞ADPase酶组化染色。结果：间接检眼镜观察发现17只SD大鼠在结束高氧环境1-3d，周边部视网膜血管呈毛刷状、无血管网，5d视网膜周边部开始出现血管网并有出血；2mo后，视网膜血管染色显示：视网膜毛细血管密度增加，血管扩张、管壁变薄、迂曲并有大量血管芽。结论：可变氧环境下形成ROP模型成型率可达66.7%，间接检眼镜检查法可排除非ROP模型。

AIM：To develop retinopathy of prematurity（ROP）model in newborn SD rats in hyperoxia circumstance for animal experiment.METHODS：Twenty-four SD rats and two mother rats were bred in oxygen container in which the oxygen concentration was set alternately 400mL and 800mL in a 12-hour circle for 14 days.Then the rats were moved into the room with normal air circumstance.The fundus of these rats were observed with indirect ophthalmoscopy.ADPase staining of blood vessel endothelium cells showed the change in retina blood.RESULTS：At 3 days after moving out of hyperoxia circumstance,the SD rats peripheral retina blood vessels were brushlike without vascular network.At 5 days after moving out of hyperoxia circumstance,vascular network was found around the blood vessel with bleeding.At 2 months after moving out of hyperoxia circumstance,ADPase staining showed both volume and density of blood vessel increased.CONCLUSION：The success rate of developing ROP model in SD rats in variable oxygen concentration is 66.7%.Non-ROP model could be excluded by using indirect ophthalmoscopy.