乙型肝炎患者血清趋化因子CXCL16的检测及其意义

The assay and significance of CXCL16 in serum of hepatitis B patients.

目的探讨乙肝患者血清趋化因子CXCL16的变化规律及其临床意义。方法用酶联免疫吸附法(ELISA)检测血清CXCL16浓度;荧光定量聚合酶链反应法(FQ-PCR)检测HBV-DNA;全自动生化仪检测肝功能。结果对照组(n=25)、急性乙肝组(n=24)、慢性乙肝组(n=32)CXCL16浓度分别为1.676±0.766(ng/ml)、2.150±0.714(ng/ml)、2.417±0.537(ng/ml)。急、慢性乙肝组CXCL16浓度显著高于对照组(P值分别为0.015、0.000)。急、慢性乙肝组之间比较,差异无统计学意义(P=0.142)。HBeAg阳性乙肝组(n=26)与HBeAg阴性乙肝组(n=30)比较,差异无统计学意义(P=0.741)。CXCL16浓度与ALT水平无相关性(r=-0.46,P=0.736),与HBVDNA拷贝数也无相关性(r=-0.191,P=0.158),但CXCL16有随病情加重而增高的趋势。结论CXCL16可能参与了乙肝的炎症损伤机制;ALT、HBVDNA及HBeAg与CXCL16浓度变化无明显相关。

Objective To investigate the change and clinical significance of serous chemokine CXCL16 in patients with hepatitis B. Methods The serous concentration of CXCL16 was detected by enzyme linked immunosorbont assay （ELISA） ; HBV - DNA was detected by fluorescent quantitive polymerase chain reaction （ FQ - PCR） ; Liver function was assayed by automatic biochemistry analyzer. Results Concentration of serous CXCL16 in normal group（ n = 25 ） ,AHB（ acute hepatitis B） group （ n =24） and CHB（ chronic hepatitis B） group （ n = 32 ） was 1. 676 ± 0. 766 （ng/ml） ,2. 150 ± 0. 714（ng/ml） and 2. 417±0. 537 （ng/ml） respectively. The concentration of CXCL16 in AHB and CHB groups were both significantly higher than that of normal group, （P = 0. 015, 0. 000, respectively）. However, the disparity of CXCL16 con- centration between AHB and CHB group isnt statistically significant （P =0. 142 ）. Likewise, no statistical significance of the difference of CXCL16 was found between HBeAg positive （ n = 26 ） and negative （ n = 30） patients group （ P = 0. 741 ）. There is no correlation between the concentration of CXCL16 and the level of ALT （r = -0. 46, P = 0. 7365 ）, so as the as- sociation of CXCL16 and HBVDNA （r = -0. 191 ,P =0. 158）. Nevertheless, the concentration of CXCL16 is raised as the pathogenetic condition aggravated. Conclusion It is possible that CXCL16 play a role in liver inflammatory in hepatitis B. The change of the serous concentration of CXCL16 isnt evidently related with that of ALT,HBVDNA and HBeAg.