光照疗法诱导新生儿氧化应激反应的研究

A study on oxidative stress in neonates with phototherapy

目的探讨光照疗法（光疗）对高胆红素血症新生儿氧化应激反应的诱导效应。方法将60例非溶血性高胆红素血症新生儿随机分为两组：光疗1组30例，入院后持续光疗24h；光疗2组30例，入院24h内不予光疗。用比色法测定两组患儿的血清丙二醛（MDA）浓度，血清超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）活性。结果光疗1组患儿光疗24h后血清MDA浓度为（18．59±3．11）nmol／ml，明显高于光疗前[（16．13±3．12）nmol／ml]，差异有非常显著性（P〈0．01）；血清SOD、CAT、GSH-Px活性分别为（63．92±12．40）U／ml、（1．60±0．44）U／ml与（74．33±31．48）酶活力单位，明显低于光疗前[（75．80±13．78）u／ml、（1．87±0．51）u／ml与（90．52±29．88）酶活力单位]，差异有非常显著性（P均〈0．01）。光疗2组患儿一般治疗24h后血清MDA浓度，,SOD、CAT、GSH-Px活性与治疗前相比，差异无显著性（P均〉0．05）。结论光疗可诱导新生儿氧化应激反应。

Objective To explore whether phototherapy induces an oxidative stress in neonates with hyperbilirubinemia. Methods Sixty neonates with hyperbilirubinemia were randomly divided into two groups, group one（30 cases） and group two（30 cases）. Patients of group one were given with 24 hours of continuous phototherapy after admission. Patients of group two were not given with phototherapy within 24 hours and only given with routine treatment. And then, we measured the level of malondialdehyde（MDA） and serum activities of superoxide dismutase（SOD）, catalase（CAT） and glutathione peroxidase（GSH-Px） by chromatometry. Results In group one, the serum level of MDA after 24 hours of continuous phototherapy was significantly higher than that before phototherapy （18.59±3.11 vs 16.13±3.12） nmol/ml（P〈0.01）, the serum activities of SOD, CAT and GSH-Px were significantly lower than those before phototherapy [（63.92±12.40 vs 75.80±13.78） U/ml, （1.60±0.44 vs 1.87±0.51） U/ml, （74.33±31.48 vs 90.52±29.88）unit of activity] （P〈0.01 ）. In group two, the serum level of MDA and the activities of SOD, CAT and GSH-Px were not different between before and after 24 hours of routine treatment （P〉0.05）. Conclusion Phototherapy induces an oxidative stress in neonates.