摘要
本文以春兰(Cymbidium goeringii)继代的根状茎为试材,研究简化实验技术手段,为春兰种苗工厂化生产提供参考。采取改变培养基中活性炭和糖分用量,不同光照、固体与液体静置培养方法,结果表明:活性炭对根状茎增殖起促进作用,但在根状茎诱芽中有强烈的抑制作用。糖分对根状茎的增殖有益,浓度宜为20g/L,可用白糖代替蔗糖。光照有利于根状茎增殖,双支日光灯照明优于散射光和黑暗培养。根状茎增殖在固体培养基中比薄层液体静置培养好,而诱芽阶段反之。
Taking the rootstocks of Cymbidium goeringii as test pieces , the paper studies the means to simplify experimental technique,in order to provide reference to the factorization produces of Cymbidium goeringii. The dosages of active carbon and carbohydrate were changed , and under varying illumination, stationary culture was carried out in both solid and liquid culture medium. It proved that: active carbon can promote the growth of rootstock,while for young sprouts in the rootstock,it has restraining effect. Carbohydrate is beneficial to the proliferation of rootstock, 20g/l is the appropriate concentration, and sucrose can be replaced by white sugar. Illumination is in favor of rootstock proliferation , that of two - branch sunlight lamp gaining advantage over scattered light and dark culture. Rootstock proliferation in solid medium get better than in shallow - layer liquid stationary culture, but on the contrary in the phase of sprout.
出处
《安徽农学通报》
2008年第7期33-35,共3页
Anhui Agricultural Science Bulletin
关键词
中国兰
春兰
组织培养
简化试验
China orchid
cymbidium goeringii
tissue culture