春兰根状茎增殖与诱芽技术研究

Study on the proliferation and sprout of cymbidium goeringii in tissue culture

本文以春兰(Cymbidium goeringii)继代的根状茎为试材,研究简化实验技术手段,为春兰种苗工厂化生产提供参考。采取改变培养基中活性炭和糖分用量,不同光照、固体与液体静置培养方法,结果表明:活性炭对根状茎增殖起促进作用,但在根状茎诱芽中有强烈的抑制作用。糖分对根状茎的增殖有益,浓度宜为20g/L,可用白糖代替蔗糖。光照有利于根状茎增殖,双支日光灯照明优于散射光和黑暗培养。根状茎增殖在固体培养基中比薄层液体静置培养好,而诱芽阶段反之。

Taking the rootstocks of Cymbidium goeringii as test pieces , the paper studies the means to simplify experimental technique,in order to provide reference to the factorization produces of Cymbidium goeringii. The dosages of active carbon and carbohydrate were changed , and under varying illumination, stationary culture was carried out in both solid and liquid culture medium. It proved that： active carbon can promote the growth of rootstock,while for young sprouts in the rootstock,it has restraining effect. Carbohydrate is beneficial to the proliferation of rootstock, 20g/l is the appropriate concentration, and sucrose can be replaced by white sugar. Illumination is in favor of rootstock proliferation , that of two - branch sunlight lamp gaining advantage over scattered light and dark culture. Rootstock proliferation in solid medium get better than in shallow - layer liquid stationary culture, but on the contrary in the phase of sprout.