旋毛虫对BALB/c小鼠体内人结肠癌HCT-8细胞的作用

Effect of Trichinella on Growth of Human Colorectal Caricnoma HCT-8 Cells in BALB/c Mice

目的观察旋毛虫感染对BALB/c小鼠体内人结肠癌HCT-8细胞生长的作用。方法用不同剂量的旋毛虫感染BALB/c小鼠,并在不同时间接种HCT-8细胞,荷瘤20 d后解剖小鼠,测定小鼠体内肿瘤的体积、重量,计算抑瘤率,并检测T淋巴细胞亚群。结果各实验组小鼠肿瘤体积和重量均显著低于对照组,CD3+、CD4+、CD8+、CD4+/CD8+比值、未长瘤小鼠比例、小鼠死亡率和抑瘤率均显著高于对照组;先接虫后荷瘤组抑瘤效果优于先荷瘤后接虫组。结论旋毛虫对BALB/c小鼠体内的人结肠癌HCT-8细胞的生长有一定的抑制作用。

Objective To observe the effect of infection with trichineila on the growth of human colorectal carcinoma HCT-8 cells in BALB/c mice. Methods BALB/c mice were infected with trichinella at various dosages and inoculated with HCT-8 ceils on different days before or after infection. Dissect the mice 20 d after inoculation,measure the size and weight of tumors to calculate the tumor-inhibiting rate,and determine the T lymphocyte subgroup. Results Both the size and weight of tumors of mice in test group were significantly lower than those in control group. However, the numbers of CD3^＋ , CD4^＋ and CD8^＋ cells, the ratio of CD4^＋ ceils to CD8^＋ ceils, the percentage of tumor-free mice as well as the death rate and tumor-inhibiting rate in test group were significantly higher than those in control group. The tumor-inhibiting effect of mice infected with trichineila before inoculation with HCT-8 cells was better than those infected after inoculation. Conclusion Trichineila showed a certain inhibiting effect on the growth of HCT-8 ceils in BALB/c mice.