血脂康对新生大鼠心肌成纤维细胞增殖及胶原合成的影响

Effect of Blood Fat Regulator Xuezhikang on Proliferation and Collagen Synthesis of Cardiac Fibroblast of Newborn Rats

目的观察血脂康对血管紧张素Ⅱ(AngⅡ)诱导的新生大鼠心肌成纤维细胞(CFs)增殖及胶原合成的影响,并探讨可能的分子机制。方法采用胰酶消化法分离培养Sprague-Dawley大鼠心肌成纤维细胞,建立AngⅡ诱导CFs增殖的模型,以MTT比色法和流式细胞仪检测细胞周期分析法观察血脂康对CFs数目和细胞周期的影响。AngⅡ及不同浓度血脂康作用48 h后,用天狼星红染色法检测培养上清中胶原的含量;ELISA法检测细胞培养上清液中TGF-β1蛋白表达;RT-PCR法检测胶原和TGF-β1 mRNA表达。结果AngⅡ对CFs增殖有明显促进作用,血脂康可明显抑制AngⅡ诱导的CFs增殖,且呈剂量依赖性;血脂康可增加G0/G1期细胞百分率,降低S、G2/M期细胞百分率,降低胶原含量、TGF-β1蛋白及胶原和TGF-β1 mRNA的表达。结论血脂康能抑制AngⅡ诱导的CFs增殖和胶原的产生,其作用可能是通过抑制TGF-β1表达实现的。

Objective To observe the effect of Xuezhikang, an extract of cholesfin, on the proliferation and collagen synthesis of cardiac fibroblasts （CFs） caused by aagiotensin Ⅱ （Ang Ⅱ ） in newborn rats and explore the potential molecular mechanism of the effect. Methotis Isolate the CFs of newborn Sprague-Dawley rats by trypsin digestion method,and induce the proliferation of CFs by AngH . Observe the effect of Xuezhikon the number and cell cycle of CFs by MTT method and flow cytometry. After treatment with Ang H and Xuezhikang at various concentrations for 48 h , the field of collagen in culture supematant was determined by Sirius staining, and the expression of TGF-β1 protein by EIJSA. The expressions of collagen gene and TGF-β1 mRNA were determined by RT-PCR. Results Ang H promoted the proliferation of CFs significantly.However, X ueddkang showed significant dose-dependent inhibitory effect on proliferation of CFs induced by AngH . The drug increased the percentage of cells at C0/G1 phase,decreased those at S and G2/M phases, and decreased the expressiom of collagen gene and TGF-β1 mRNA. Conclusion Xuezhikang inhibited the proliferation of CFs and production of collagen induced by Ang Ⅱ . The mechanism for this might be the inhibition of TGF-β1 expression.