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重组人粒细胞集落刺激因子的表达、纯化以及PEG修饰 被引量：2

Expression,purification and PEGylation of recombinant human granulocyte colony-stimulating factor
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摘要 重组人粒细胞集落刺激因子(rhG-CSF)经大肠杆菌温度诱导表达后,其表达产物以包涵体形式存在,包涵体经过变性、复性和分离纯化等步骤处理后得到纯化的rhG-CSF。在一定的实验条件下用单甲氧基聚乙二醇活性酯(mPEG20k-NHS)对rhG-CSF进行化学修饰,所得修饰产物经分离纯化后获得PEG20K-rhG-CSF偶联物。与修饰前的rhG-CSF相比较,尽管修饰后的rhG-CSF体外生物学活性下降至修饰前的20%左右,但其在体内的作用时间能够得到显著的延长,药效有了明显提高。 Recombinant human granulocyte colony-stimulating factor（rhG-CSF） expressed in Escherichia coli in form of inclusion body was modified by monomethoxy polythylene glycol （mPEG20k-NHS） after denaturation, renaturation and purification. The modified rhG-CSF（PEG20k-rhG-CSF） was obtained by purification of modified product. Compared with unmodified rhG-CSF, the modified rhG-CSF had 20% biologic activity of unmodified rhG-CSF in vitro, but it had significantly prolonged action time in vivo with obviously elevating pharmacodynamics.
作者 张兵 邹文艺 戎隆富 范清林 宋礼华
机构地区 安徽医科大学基础医学院 安徽安科生物工程股份有限公司
出处 《生物学杂志》 CAS CSCD 2008年第2期36-39,共4页 Journal of Biology
关键词 重组人粒细胞集落刺激因子 表达 纯化 单甲氧基聚乙二醇 生物学活性 rhG-CSF expression purification monomethoxy polythylene glycol biological activity
分类号 Q785 [生物学—分子生物学]
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参考文献9

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二级参考文献19

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生物学杂志
2008年 第2期

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