摘要
本文研究了绿色巴夫藻(Pavloca viridis)一次性培养过程中。不同氮浓度(0、100、880、7040μmol/L)和磷浓度(0、10、36.3、290.4μmol/L)对其生长及叶绿素荧光参数的影响。培养温度为20±1℃,盐度为31,光照强度为100μmol/Lolm^(-2)s^(-1)。单因子方差分析结果表明。在整个培养过程中,氮磷浓度对绿色巴夫藻的生长及各叶绿素荧光参数都有显著影响(P<0.05)。多重比较结果表明,绿色巴夫藻生长和进行光合作用的最适氮浓度为880μmol/L、磷浓度为36.3μmol/L。此条件下,该藻的细胞密度、叶绿素相对含量、F_v/F_m(PSⅡ的最大光化学效率)、ΦPSⅡ(PSⅡ的实际光能转化效率)、ETR(电子传递效率)、qP(光化学淬灭)显著高于其他浓度组。qN、NPQ(非光化学淬灭)开始处于较低水平,随着培养时间的延长逐渐增加至最高水平。
The effects of different nitrogen concentrations(0, 100, 880, 7040μmol/L) and phosphorus concentrations(0, 10, 36.3, 290. 4μmol/L)on the growth and chlorophyll fluo- rescence parameters of Pavloca viridis are reported. The Pavloca viridis was grown at 204±1℃, the salinity was adjusted to 31 by using distilled water, and the light intensity was 100μmol/Lolm^-2s^-1. One-way analysis of variance shows that nitrogen and phosphorus concentrations have significant effects on the growth and photosynthesis of Pavloca viridis during the experimental period(P〈0.05). Multiple comparison tests show that the optimal nitrogen and phosphorus concentrations for the growth and photosynthesis of Pavloca viridis are 880μmol/L and 36.3μmol/L, respectively. In this condition, the fluorescence parameters Fv/Fm(the maximal photochemical efficiency of PSII), ΦPSII(the actual photochemical efficiency of PSII), ETR(the electron transport rate), qP(the photochemical quenching)are higher than other treatments while qN and NPQ (nonphotochemical quenching)reach the highest level with increasing culture age after a significant decrease.
出处
《海洋湖沼通报》
CSCD
北大核心
2008年第1期120-128,共9页
Transactions of Oceanology and Limnology
基金
中国海洋大学海水养殖教育部重点实验室开放课题资助(200603)
关键词
绿色巴夫藻
氮浓度
磷浓度
生长
叶绿素荧光
Pavloca viridis
nitrogen concentrations
phosphorus concentrations
growth
chlorophyll fluorescence parameters