附子与桂枝配伍对大鼠佐剂性关节炎的实验研究

Experimental study of compatible application of strobal and Ramulus Cinnamomi on adjuvant arthritis rats

目的研究附子与桂枝配伍对大鼠佐剂性关节炎镇痛、抗炎、抗自由基损伤等方面的影响。方法采用弗氏完全佐剂局部注射法复制大鼠佐剂性关节炎模型,观察附子、桂枝及其不同配伍比例对佐剂性关节炎大鼠的痛阈、足跖厚度以及血清中NO、SOD、MDA含量的影响。结果单味附子、桂枝及其不同配伍比例均具有镇痛、抗炎、抗自由基损伤等方面的作用,但配伍应用后作用明显优于单味附子或桂枝,表现出明显的配伍优势。结论①附子与桂枝配伍在抗炎、镇痛、抗自由基损伤等方面具有显著的协同增效作用,揭示了其"相须"配伍的科学性。②发挥镇痛、抗自由基损伤作用的最佳配伍比例同为附子桂枝9∶4;发挥抗炎作用的最佳配伍比例为附子桂枝3∶2。

Objective It is to study the influence of compatible application of strobal and Ramulus Cinnamomi on relieving pain, anti-inflammatory and against free radical injury in adjuvant arthritis （AA） rats. Methods The AA rat models were induced with part injection of Freund＇s complete adjuvant. The influence of strobal, Ramulus Cinnamomi and their different compatible proportions on pain threshold, voix pedis thickness and the serum contents of NO, SOD and MDA in AA rats was observed. Results Strobal, Ramulus Cinnamomi and their different compatible proportions all had the effects of relieving pain, anti-inflammatory and against free radical injury. But the effect of compatible application of strobal and Ramulus Cinnamomi obviously outstripped that of strobal or Ramulus Cinnamomi only, which displayed compatible advantage. Conclusion ①Compatible application of strobal and Ramulus Cinnamomi has significantly synergistic effect on relieving pain, anti- inflammatory and against free radical injury, which reveals the scientific quale of mutual promotion compatible application. ②The best compatible proportion of strobal and Ramulus Cinnamomi on relieving pain and against free radical injury is 9 ： 4. The best compatible proportion of strobal and Ramulus Cinnamomi on anti- inflammatory is 3：2.