摘要
目的：探讨iressa对胃癌SGC.7901细胞增殖，细胞周期和细胞凋亡的影响。方法：用四氮甲唑蓝（MTT）法检测不同浓度iressa对胃癌SGC-7901细胞增殖的影响；用流式细胞仪检测不同浓度iressa对胃癌SGC-7901细胞周期分布和细胞凋亡的影响。结果：在5～20μmol／L浓度范围内，iressa对SGC-7901的生长具有明显的抑制作用，并呈时间和剂量依赖性。10μmol／L和20uol／L的iressa可以导致SGC-7901细胞G1期阻滞，并引起细胞凋亡。结论：Iressa可改变SGC-7901细胞周期分布，诱导细胞凋亡，从而抑制细胞增殖。
Objectives ：To investigate the effect of iressa on the proliferation,cell cycle and apoptosis of human gastric cancer SGC-7901 ceils. Methods：The proliferation inhibition of SGC-790 1 cells exposed to different concentration of iressa was observed by MTT assay. The cell cycle and apoptosis of SGC-7901 cells exposed to different concentration of iressa was analyzed by flowcytometry. Results：Within the rang of 5-20 umol/L,iressa obviously inhibited the proliferation of SGC-790 1 cells in a time--and dose--dependent manner. 10umol/L and20 umol/L iressa caused SGC-790 1 cells to undergo G1 arrest and induced apoptosis. Conclusion： Iressa could inhibited SGC-7901 cells proliferation,caused G1 arrest and induced apoptosis.
出处
《陕西医学杂志》
CAS
北大核心
2008年第4期459-461,共3页
Shaanxi Medical Journal
基金
陕西省攻关项目[2006K09-G6-(1)]
