海洋真菌095407的抗菌活性代谢产物的研究

Antibacterial metabolites from the fermentation broth of marine fungus 095407

目的对海洋真菌095407的抗菌活性成分进行研究。方法用多种色谱技术对化合物进行分离纯化,用光谱技术和理化性质鉴定化合物的结构,并用滤纸片琼脂扩散法测定化合物的抗耐甲氧西林金黄色葡萄球菌和白色念珠菌活性。结果从095407号真菌发酵液提取物中分离得到4个化合物,分别鉴定为3,4,5-三甲基-1,2-苯二酚(1)、decarboxydihydrocitrinone(2)、甘露醇(3)、刺芒柄花素(4)。结论化合物1为首次以天然产物的形式分离得到,活性测试结果表明化合物1和2具有抗耐甲氧西林金黄色葡萄球菌活性。

Aim To study the antibacterial constituents from marine fungus 095407. Methods The secondary metabolites were isolated and purified by column chromatography, and their structures were identified by physicochemical and spectral data. Their antibacterial activities were tested against methicillin-resistant Staphylococcus aureus （MRSA） and Candida albicans by paper disco diffusion method. Results Four compounds were isolated from the broth of marine fungus 095407, and their structures were elucidated as 3, 4, 5-trimethyl-1,2-benzenediol （ 1 ）, decarboxydihydrocitrinone （ 2 ）, mannitol （3）, and formononetin （ 4 ）. Conclusion Compound 1 was a new natural product. Compounds 1 and 2 showed inhibitory effect on MRSA.