内耳冲击波缺氧复合伤动物模型的建立

Establishment of an inner ear blast-hypoxia combined injury model in guinea pig by the bio-shock tube and hypoxia-cabin

目的建立内耳冲击波缺氧复合伤动物模型。方法采用BST-I型生物激波管致伤1次,高压段压力为50kPa。冲击波致伤后,将豚鼠放入自制动物笼,置于密闭低氧舱内,持续吸入10%低氧混合气体,共1h。观察豚鼠一般情况、听性脑干反应阈值及耳蜗组织病理变化等情况并进行比较。结果造模后1h,豚鼠精神极度萎靡,呼吸急促,反应迟钝;听性脑干反应阈值明显升高,与对照组相比较有非常显著性差异(P<0.01)。结论采用BST-I型生物激波管,高压段压力为50kPa,致伤1次,致伤后持续吸入10%低氧混合气体1h可成功复制出豚鼠内耳冲击波缺氧复合伤动物模型,可作为内耳冲击波缺氧复合伤研究的实验模型。

Objective To set up an inner ear blast-hypoxia combined injury model in guinea pig. Method The guinea pigs were inflicted with blast injury by the bio-shock tube with blast wave overpressure for 50.0 kPa. After blast injury, the guinea pigs were put into the hypoxia-cabin immediately, and the mixed gases of 10% oxygen concentrations were given. Result Posterior to the blast-hypoxia combined injury, the guinea pigs became depressed, breathlessness and clumsy, and the ABR threshold of the guinea pigs increased significantly （P〈0.01）. Conclusion Through abovementioned method a steady guinea pig inner ear blast-hypoxia combined injury model was successfuly established with the bio-shock tube and hypoxia-cabin by authors, the model could be taken as experimental model for studying the combined injuries.