Morquio A综合征的诊断研究 Ⅲ.Gal-6S的酶动力学研究

Fluorimetric Enzymatic Diagnosis of Morquio A Syndrome Ⅲ. Kinetic Studies of Galactose-6-sulphate Sulphatase

利用合成的4Mu-Ga1-6S,研究了成纤维细胞、白细胞、羊水细胞、绒毛细胞、绒毛组织和胚胎肝、脑、肌肉中Ga1-6S的酶动力学。分别观察了pH值、温浴时间、样品浓度和底物浓度对酶活性的影响。

Enzymatic kinetics of Gal-6 S was studied with prepared fluorimetric substrates in skin fibroblasts, leucocytes, amniocytes, chorionic villous cells, chorionic villi and fetal tissues (brain, liver and muscle) about PH, incubation time, protein concentration and substrate concentration.