福建青梅rDNA ITS区克隆与序列分析

ITS Sequence Analysis of Nuclear Ribosomal DNA of Prunus mume in Fujian Province

利用PCR法对青梅ITS1、5.8S、ITS2序列扩增后克隆测序,用软件DNAMAN和MEGA3.1分析测序结果,研究18个福建青梅样品的核糖体ITS碱基序列差异。获得青梅18个样品rDNA中的ITS和5.8S完全序列,ITS1、5.8S和ITS2序列长度分别为223～224bp、164bp和241～246bp,5.8S较为保守。根据测序结果,以UPGMA法建立系统发生树,从分子水平说明18个样品间的变异程度,并将福建青梅差异较大的14条rDNA ITS序列登录GenBank,获得登录号:EF523482-EF523493、EF529435和EF529436。

To study the ITS sequence variation ofPrunus mume in Fujian province from different population, the rDNA ITS regions of P. mume varieties were amplified and cloned and sequenced, and sequence data was analyzed by DNAMAN and MEGA3.1. The complete sequence of ITS and 5.8S rDNA of 18 different P. mume population were obtained. The sequences of ITS1, 5.8S and ITS2 are 223-224 bp, 164 bp and 241-246 bp, respectively, 5.8S is more conservative than ITS. Phylogenetic tree based on ITS sequences data was conducted by UPGMA method, which shows the variation at the level of molecular biology. It provided the basis for variety identification ofP. mume And 14 rDNA ITS sequences of P. mume were deposited in GenBank under number EF523482-EF523493, EF529435 and EF529436.