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呼吸道合胞病毒性婴幼儿肺炎快速诊断的研究

Rapid Diagnosis of RSV Pneumonia
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摘要 我们应用抗呼吸道合胞病毒单克隆抗体(RSV McAb)免疫荧光法,检查了144例临床诊断为婴幼儿病毒性肺炎患儿咽部脱落细胞中的RSV特异性抗原,同时对部分病例做了病毒分离和(或)双份血清抗体测定对照检查,结果McAb荧光抗体法阳性率为58.4%(84/144),明显高于后两种方法(P值分剐为<0.01和<0.05),而且达到了特异敏感的要求。 With McAb against RSV were detected RSV specific Ag in pharynx exfoliative cells of 144 patients who were diagnosed as infant viral pneumonia. Virus isolation and doble-serum antibody assay were made as controls in some cases. The results showed that the positive rate of immunofluorescence was 58.4% (84/ 144), and that of virus isolation was 40.8% (58/142). The difference was significant between them, ≥4 times in double serum antibody assay were 68.3% (43/63). RSV Ag in exfoliative cells were detected with McAb in 56 of 63. The possitive rate was 82.1% (46/56). The positive rate of latter was much higher than that of double serum antibody examination, P<0.05. So this method could be used in rapid diagnosis of RSV pneumonemia.
出处 《哈尔滨医科大学学报》 CAS 1990年第4期230-232,共3页 Journal of Harbin Medical University
关键词 RSV 肺炎 合胞病毒 诊断 儿童 Respiratory syncytial virus Monoclonal antibody indirect immunofluorescence test Pneumonia
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