摘要
目的建立血清中地西泮的高效液相色谱测定方法。方法以甲醇-水(70:30)为流动相;Kromasil C18(250mm×4.6mm5μm)柱为层析柱,以卡马西平为内标物,检测波长216nm。结果地西泮在0.1~20.0mg·L^-1浓度范围内具有良好的线性关系,平均回收率为97、67%,日内、日间RSD小于4.8%,最低检测浓度为20μg·L^-1。结论本方法简便可行,适合于地西泮的血药浓度测定。
Aim To eatablish an HPLC method for the determination of diazepam in serum. Methods Mobile phase consisted of metha- nol-water(70:30 ). Kromasil C is (250 mm × 4.6 mm 5 μm) column was used,with carbamazepine as internal standard, and UV-detector wavelength was 216 nm. Results The linear range was between 0. 1 and 20.0 mg · L^-1. The average recovery was 97.67%, and RSD of within-day and between-day was less than 4.8%. Detection limits were above 20 ng · m L^-1. Conclusion This method is simple and feasible for the determination of diazepam in serum.
出处
《安徽医药》
CAS
2008年第4期319-320,共2页
Anhui Medical and Pharmaceutical Journal