罗格列酮对糖尿病大鼠肾组织nephrin表达的影响

Effect of rosiglitazone on the expression of nephrin in the kidney of diabetic rats

目的:探讨糖尿病大鼠肾组织nephrin的表达以及罗格列酮干预后对其表达的影响。方法:链脲佐菌素(STZ)诱导糖尿病大鼠模型,将糖尿病大鼠随机分为糖尿病非干预组(DM组)和罗格列酮干预组(DR组),并以正常组(NC组)作对照。干预8周后,观察各组大鼠尿蛋白、血糖、血脂、血尿素氮(BUN)、血肌酐(Cr)变化;用免疫组化及实时荧光定量PCR检测肾组织nephrin蛋白及mRNA的表达。结果:①DM组大鼠尿蛋白、血BUN,Cr较NC组明显升高,肾组织nephrin蛋白及mRNA表达降低;与DM组相比,DR组大鼠尿蛋白、血BUN,Cr降低,nephrin蛋白及mR-NA表达上调。②DM组血糖、血脂与NC组相比,差异有统计学意义,与DR组比较差异无统计学意义。结论:罗格列酮可能通过上调糖尿病大鼠肾组织nephrin表达,发挥不依赖糖脂代谢的直接肾保护作用。

Objective. To investigate the effect of rosiglitazone on the expression of nephrin in the kidney of diabetic rats. Methods： Diabetic model was established by a single injection of streptozotocin（ STZ）, and diabetic rats were randomly divided in to 2 groups ： untreated diabetes mellitus group （ DM group） and rosiglitazone-treated diabetes mellitus group （DR group）. In addition, the normal rats served as a normal control group （NC group）. Eight weeks later, the excretion of urine protein, blood glucose, serum lipid profile, serum urea nitrogen and serum creatinine were detected. The expression of nephrin was detected by immunohistochemistry and real-time Q-PCR. Results： Compared with NC group, the excretion of urine protein, serum urea nitrogen and serum creatinine in DM group were significantly increased, and the expression of nephrin was significantly down-regulated. Compared with DM group, the excretion of urine protein, serum urea nitrogen and serum creatinine in DR group were significantly decreased, and the expression of nephrin was significantly up-regulated. Blood glucose and serum lipid profile were significantly changed in DM group compared with NC group, while they had no significant difference between DM group and DR group. Conclusion： Rosiglitazone may up-regulate the expression of nephrin in the kidney of diabetic rats to produce direct nephroprotective effects independent of glucose and lipid metabolism.