摘要
目的:研究食管癌患者转录因子T-bet,GATA-3的基因表达及其对患者免疫状态的影响。方法:根据其CT分期将40例食管癌患者分为两组:一组为第Ⅰ,Ⅱ期患者,另一组为第Ⅲ,Ⅳ期患者;荧光定量PCR技术检测40例食管癌患者及20例健康对照者外周血单核细胞T-bet,GATA-3 mRNA的表达水平;ELISA法检测血浆中辅助性T细胞1型(Th1)细胞因子IFN-γ及辅助性T细胞2型(Th2)细胞因子IL-4。结果:食管癌组较对照组T-bet mRNA表达明显降低,GATA-3 mRNA表达明显升高,IFN-γ表达明显降低,IL-4表达明显升高,不同期别食管癌组间差异不显著。T-bet,GATA-3分别与IFN-γ,IL-4呈正相关。结论:食管癌患者Th1型细胞因子表达降低,Th2型细胞因子表达升高,即Th1/Th2平衡被打破,发生了向Th2方向的漂移,免疫功能受损;这种改变受到转录因子T-bet,GATA-3的调控,T-bet表达的减少在这种调控中可能起着关键的作用。
Objective： To evaluate the expression of transcription factors T-bet/GATA-3 mRNA in patients with esophageal carcinoma and their influence on those patients＇ immunity. Methods： According to CT image,patients were divided into two groups, stage I plus II, and, stage m plus IV; The gene expression of T-bet/GATA-3 in patients with esophageal carcinoma and control group was detected by reverse transcription-polymerase chain reaction RT-PCR. The expression of IFN-γ and IL-4 for Thl/Th2 cytokine was measured by ELISA. Results： T-bet mRNA expression of esophageal carcinoma was significantly lower than that in control, the expression of GATA-3 mRNA in esophageal carcinoma was significantly enhanced. The plasma level of Thl cytokine IFN-γ in esophageal carcinoma was significantly lower than that in control. The level of Th2 cytokine IL-4 in esophageal carcinoma was significantly higher than that in control. However, the difference between two groups was not significant. Conclusion： In patients with esophageal carcinoma, we found there were lower production of IFN-γ for Thl and over-production of IL-4 for Th2, which meant there was imbalance between Thl and Th2. The change that the imbalance differentiation of Th cells was regulated by T-bet and GATA-3 ,and the less T-bet expression may be a more important factor.
出处
《江苏大学学报（医学版）》
CAS
2008年第2期156-158,162,共4页
Journal of Jiangsu University：Medicine Edition
基金
江苏大学研究生创新基金资助项目(1291280010)
