葛根异黄酮柱分离纯化技术研究

Study on Isolation and Purification of Coloum Technology for the Isoflavones of Kudzu Root

以葛根异黄酮含量为44.2%的葛根粗提物为原料,通过对聚酰胺(13～40目)、AB—8、HP—20三种树脂对葛根异黄酮静态吸附与解吸的比较,在筛选出一种具有较高吸附率与解吸率的树脂的基础上,系统优化了此种树脂柱分离纯化葛根异黄酮的技术参数。结果表明,静态吸附与解吸时,HP—20树脂最适合于葛根异黄酮分离纯化,吸附率、解吸率分别达57.53%、37.49%。HP—20树脂柱分离纯化葛根异黄酮的最优技术参数为:50%乙醇洗脱,流速0.9BV/h,3BV的洗脱液。在此工艺条件下,葛根异黄酮的洗脱率、纯度和得率分别达53.67%、55.78%和44.70%。

By compared the adsorption and adsorption efficiency of isoflavones of Kudzu root with PA, AB-8, HP-20 ,discover the type of HP-20 has a preferably performance of the adsorption and de-sorption to the isoflavones of Kudzu root.. The ad-sorption of HP-20 is high to 57.53% ,the de-sorption is 37.49. Because of it ,we optimize the parameter of dynamic technics . Compared the results, we could conclude that the best parameter of technics： with 50% ethanol de-sorption under the velocity of 0.9BV/h ,the bulk of de-sorption is 3BV. Under this condition, the rate of de-sorption purification and production are exceed 53.67%,55.78% and 44. 70%.