HBV感染患者外周血T淋巴细胞CD_(25)的表达及意义

CD_(25) Expression in Peripheral Blood T Lymphocytes from Patients with Chronic Hepatitis B and Its Significance

目的观察乙型肝炎患者外周血T淋巴细胞CD25表达,探讨CD25在慢性乙型肝炎发病中的意义。方法采用流式细胞检测技术,检测慢性乙型肝炎患者外周血T淋巴细胞CD25表达,观察CD25在慢性乙型肝炎患者的表达状况,同时观察了慢性重症肝炎、急性乙型肝炎患者、病毒携带者CD25表达情况。结果乙型肝炎患者T细胞存在不同程度CD25表达,CD3+T及CD4+T表达为明显,CD8+TCD25表达较低;慢性乙型肝炎患者外周血CD3+T淋巴细胞CD25表达占外周血单个核细胞(2.92±0.13)%,急性乙型肝炎患者(0.51±0.36)%,慢性重症肝炎(1.60±0.07)%,病毒携带者(0.95±0.23)%;慢性乙型肝炎患者外周血CD4+T淋巴细胞CD25表达(2.58±0.50)%,急性乙型肝炎患者(0.34±0.07)%,慢性重症肝炎(1.45±0.02)%,病毒携带者检出率(0.83±0.13)%;慢性乙型肝炎患者、慢性重症肝炎较病毒携带者、急性乙型肝炎患者相比,CD3+T及CD4+TCD25表达明显增加(P<0.05),慢性乙型肝炎患者较慢性重症肝炎相比,CD3+T及CD4+TCD25表达明显增加(P<0.05)。结论乙型肝炎患者外周血T细胞CD25主要在CD4+T细胞表达,慢性乙型肝炎患者CD4+CD25+T细胞较急性肝炎增多,提示乙型肝炎免疫耐受与CD25可能有关。

OBJECTIVE To investigate CD25 expression in peripheral blood T lymphocytes from patients with chronic hepatitis B,and explore its significance in the pathogenesis of chronic hepatitis B.METHODS To detect CD25 expression in peripheral blood T lymphocytes of patients with chronic hepatitis B by means of flow cytometry.CD25 expression was observed in chronic hepatitis B patients.In the meantime,CD25 expression in T cells from severe chronic hepatitis or acute hepatitis B patients and asymptomatic carriers of HBV was also observed.RESULTS Varying degrees of CD25 were expressed in T cells from hepatitis B patients.The expression in CD3^＋ T and CD8^＋T cells was higher than that in CD4^＋T cells.CD25 expression in CD4^＋T was lower.The average of CD25 expression in CD3^＋T cells from patients with chronic hepatitis B,acute hepatitis B,and chronic severe hepatitis B and asymptomatic carriers of HBV was（2.92±0.13）%,（0.51±0.36）%,（1.60±0.07）%,and（0.95±0.23）%,respectively.The average of CD25 expression in CD4＋T cells from patients with chronic hepatitis B,acute hepatitis B and chronic severe hepatitis B and asymptomatic carriers of HBV was（2.58±0.50）%,（0.34±0.07）%,（1.45±0.02）%,and（0.83±0.13）%,respectively.CD3^＋T and CD4^＋T CD25 expression in patients with chronic hepatitis and,severe chronic hepatitis B was increased compared with that of acute hepatitis B patients and asymptomatic carriers of HBV.Compared with chronic severe hepatitis B,the expression of chronic hepatitis B was higher.CONCLUSIONS CD4^＋ CD25^＋T cells in chronic hepatitis B virus infection are increased compared with acute hepatitis,CD4^＋ CD25^＋T cells may be related to immune tolerance.