新疆野猪mtDNA控制区序列遗传多样性分析

Genetic Diversity Analysis of mtDNA Control Region Sequence in Xinjiang Wild Pig

[目的]为研究猪种起源和遗传分化提供依据。[方法]从6个新疆野猪样本中提取基因组DNA,克隆线粒体DNA控制区序列并进行测序。在测定新疆野猪与其他20个国内外猪种间的遗传距离的基础上,对其进行聚类分析。[结果]通过PCR扩增克隆到大小为1398bp的DNA片段,其A+T含量为59.87%,有明显的碱基偏向性。控制区内存在大量的ACACGTGCGT串联重复序列。在6个新疆野猪样本中检测到3个单倍型。群体平均单倍型多样度(H)为0.600,核苷酸多样度(π)为0.00086。聚类分析结果表明新疆野猪与滇南小耳猪、泰国野猪和藏猪具有较近的遗传关系。[结论]新疆野猪与亚洲野猪和中国地方猪种有较近的遗传关系。

[Objective] The research aimed to provide basis for studying the origin and the genetic differentiation of pig species. [Method] Genomic DNA was extracted from 6 samples of Xinjiang wild pig. The mitochondrial DNA control region sequence was cloned and sequenced. Based on determining the genetic distances between Xinjiang wild pig and other 20 pig species at home and abroad, the clustering analysis was made on them. [Result] DNA fragment with the size of 1398 bp was cloned by PCR amplification, with A＋T content of 59.87%, and it had an obvious base bias. There were a lot of ACACGTGCGT tandem repeat sequences in control region. 3 haplotypes were detected in 6 samples of Xinjiang wild pig. The average haplotype diversity （H） and the nucleotide diversity of the population were 0.600 and 0.00086 resp. The results of clustering analysis showed that Xinjiang wild pig had closer relationships with Diannan small-ear pig, Thailand wild pig and Tibetan pig. [Conclusion] Xinjiang wild pig had closer relationships with Asian wild pig and Chinese local pig species.