罗汉果的组织培养与快速繁殖

Study on the Tissue Culture and Rapid Propagation of Siraitia Grosvenorii

[目的]进一步探讨罗汉果高效再生系统的建立条件。[方法]通过研究外植体苗龄、激素组合等因素对罗汉果离体繁殖影响,建立了罗汉果高效再生系统。[结果]3-9 d苗龄子叶与不定芽分化的关系研究表明,在3-6 d内,子叶不定芽的分化率随着苗龄的增加而增加,以6 d的子叶出芽效果最好（83.3%）,随后不定芽分化率逐渐下降。BA和IBA不同浓度组合对子叶不定芽诱导的影响表明,在MS＋BA 1.0 mg/L＋IBA 0.5 mg/L＋3%蔗糖＋0.65%琼脂（pH值5.8）培养基上子叶外植体不定芽的诱导率达到85.7%;在1/2 MS＋IBA1.0 mg/L液体培养基上无菌苗发根率可达91.7%。抗生素敏感性试验表明,当培养基中添加10和20 mg/L的潮霉素即能完全抑制不定芽和不定根的分化。[结论]IBA与BA配合使用对罗汉果不定芽的分化有明显的促进作用。

[Objective] The aim of the research was to disenss the conditions for establishing the efficient regeneration system of Siraitia grosvenorii further. [Method] Based on studying the effects of factors such as the seedling age of explant, hormone combinations on the propagation in vitro, the efficient regeneration system of S. grosvenorii was established [ Result ] The research on the correlation between cotyledon with the seedling age of 3 - 9 d and the adventitious bud differentiation showed that the differentiation rate of adventitious bud in cotyledon was increased with the increasing of the seedling age within 3 - 6 d . The germination effect of cotyledon with the seedling age of 6 d was best （reaching 83.3% ）and then the differentiation rate of adventitious bud was gradually decreased. The research of the effects of combination of BA and IBA with different concn, on the adventitious bud differentiation of cotyledon showed that the differentiation rate of adventitious bud in cotyledon explant on the medium of MS ＋ BA 1.0 mg/L ＋ IBA 0.5 mg/L ＋ 3% sucrose ＋0.65% agar （ pH value of 5.8） reached 85.7%. The rooting rate of aseptic seedlings on the liquid medium of 1/2MS and IBA 1.0 mg/L could reach 91.7%. The antibiotic sensitivity test showed adding 10 and 20 mg/L hygromycin to the medium could inhibit the differentiation of adventitious buds androots. [ Conclusion] The combination use of IBA and BA had an obvious promotion effect on the differentiation rate of adventitious bud in S, grosvenorii .