不同培养基组分对乌桕组培植株生长与生根的影响

Effects of Different Medium Components on the Growth and Rooting of Sapium sebiferum(Linn.) Roxb.

[目的]寻求适合乌桕生长与生根的最佳培养基组分,以提高乌桕组培苗的成活率。[方法]以中国原产乌桕试管苗为材料,WPM为基本培养基,添加不同浓度的激素(IAAI、BA、NAA、6-BA、KT),组配成11种不同组分的培养基,比较研究不同培养基组分对乌桕植株生长与生根的影响。[结果]在11种培养基中,以附加10 g/L蔗糖8、g/L琼脂、Vc 5 mg/LI、AA 0.3 mg/LI、BA 0.5 mg/L、NAA 0.2 mg/L组分的培养基效果最佳,其组培苗的成活率为95.00%,生根率55.36%,株高(13.17±1.52)cm,根数目(14.01±0.52)根,侧根数目(13.01±0.51)根,主根长度(5.83±0.19)cm,是本研究中乌桕组培苗植株健壮生长与根生长最快的培养基。[结论]附加0.2%活性炭不利于乌桕的组培苗植株生长和根发育。

[ Objective] The research aimed to seek the optimum medium components for the growth and rooting of Sapium sebiferum （Linn.） Roxb. and increase the survival rate of tissue culture seedlings of S. sebiferum. [Method] With the test-tube seedling of S. sebiferum originally produced in China as materials, taking WPM as basic medium, different concn, of hormones （IAA, IBA, NAA, 6-BA,KT）were added to matched into 11 kinds of medium with different components. And the effects of different medium components on the plant growth and rooting of S. sebiferum were studied and compared. [ Result] Among 11 kinds of medium, the effect of medium with adding components of 10 g/L sucrose, 8 g/L agar, 5 mg/L Vc,0.3 mg/L IAA,0.5 mg/L IBA,0.2 mg/L NAA was best, on which the survival rate of tissue culture seedlings was 95.00%, the rooting rate was 55.36% , plant height was （ 13.17 ± 1.52 ）cm and the root number, the number of lateral roots and the length of main roots were （ 14.01 ± 0.52）, （ 13.01 ± 0.51 ） and （5.83 ± 0.19） cm resp. It was the medium on which the plants of tissue culture seedlings of S. sebiferum grew vigorously and the roots grew fastest in this research. [Conclusion] Adding 0.2% activated carbon was unfavorable for the plant growth and the development of roots in tissue culture seedlings of S. sebiferum.