良性前列腺增生MSCT多期增强特征与VEGF、bFGF表达和血管生成关系的探讨

Correlative study of VEGF,bFGF and angiogenesis with MSCT multi-phase scan features of benign prostatic hyperplasia

目的:探讨良性前列腺增生(BPH)的MSCT多期扫描强化特征与VEGF、bFGF及微血管生成之间的关系。材料和方法:对经病理证实的35例BPH患者术前行MSCT平扫和增强扫描,用免疫组化检测增生组织的VEGF、bFGF的表达以及MVD和微血管相关指标表达情况,将MSCT强化特征与免疫组化结果进行分析。结果:MSCT多期动态增强扫描见BPH强化峰值71.43%(25/35)位于延迟期(180s),28.57%(10/35)位于静脉期(75s);VEGF、bFGF的阳性表达率分别为31.43%(11/35)和14.29%(5/35)。MVD与VEGF、bFGF有相关性(P<0.01);MAV与MVD、VEGF、bFGF亦有相关性(P<0.01),与微血管的平均直径、平均周长、平均面积呈负相关(P<0.01),与微血管的异型指数无相关性(P>0.01);VEGF与bFGF的表达之间具有相关性(P<0.01);结论:bFGF、VEGF及MVD在BPH呈现不同程度的表达,MSCT强化特征与增生组织中bFGF、VEGF的表达及微血管的生成关系密切。

Objective： To investigate the correlation of the expression of vascular endothelial growth factor （VEGF）, basic fibroblast growth factor （bFGF） and angiogenesis with MSCT features of benign prostate hyperplasia （BPH）. Materials and Methods： Thirty-five BPH cases proved by histopathology were examined with muhi-phase contrast-enhanced MSCT scanning. Each pathologic specimen was stained with hemetoxylin and eosin and immunohistoehemieal technique to detect the level of MVD, VEGa, bFGF and related mierovascular indexes. The CT features and the expression of MVD, VEGF, bFGF were analyzed to investigate the relationship with each other. Result： The peak value of 71.43%（25/35） BPH was seen in delayed phase（180s）, 28.57%（10/35） in venous phase（75s）. The positive rate of VEGF and bFGF was 31.43%（11/35） and 14.29%（5/35） respectively. There was significant positive correlation between VEGF, bFGF and MVD （P〈0.01）, and between MAV and MVD, VEGF, bFGF （P〈0.01）, between VEGF and bFGF （P〈0.01）, and negative correlation of MAV with average diameter, perimeter and area. There was no correlation between MAV and heterotypie index （P〉0.01）. Conclusion： The expression of bFGF, VEGF and MVD in BPH was different. The MSCT muhi-phase scan features of BPH have significant correlation with the expression of VEGF, bFGF and angiogenesis.