基质金属蛋白酶-9和白介素9在急性毛细支气管炎发病中作用的研究

Study on the role of interleukin-9 and MMP-9 in the pathogenesis of acute bronchiolitis

目的探讨急性毛细支气管炎(简称毛支)气道中中性粒细胞、白介素9(IL-9)和基质金属蛋白酶-9(MMP-9)在其发病中的作用。方法以2006年11月至2007年4月在常州市儿童医院诊断为急性毛支的41例患儿和对照组8例无喘息的病毒性肺炎患儿为研究对象,其中急性毛支患儿根据入院Lowell评分(≥10分为重度,<10分为轻度)分为重度毛支组(10例)和轻度毛支组(31例)。对所有毛支患儿在急性期和缓解期均进行诱导痰的细胞分类检查,并用ELISA方法检测痰液中的IL-9和MMP-9,对照组进行相同操作。结果急性毛支患儿诱导痰细胞中中性粒细胞明显升高,在急性发作期明显高于缓解期和对照组;其缓解期中性粒细胞数明显高于对照组。急性毛支患儿痰中MMP-9质量浓度在急性期和缓解期与对照组比较差异亦均有统计学意义。缓解期的IL-9与对照组比较差异有统计学意义。重度毛支组急性期与缓解期比较MMP-9差异有统计学意义;轻度毛支组急性期与缓解期比较IL-9差异有统计学意义。结论中性粒细胞和MMP-9在急性毛支的发病中起重要作用,中性粒细胞和MMP-9的表达与毛支病情的严重程度相关。

Objective To investigate the role of neutrophil,interleukin-9 and MMP-9 in acute bronchiolitis.Methods Sputum was induced in 41 cases of acute bronchiolitis and 8 cases of viral pneumonia.The 41 cases of bronchiolitis were divided into two groups according to Lowell grading.Interleukin-9 and MMP-9 were montiered in each group.Results The number of neutrophil in the induced sputm of acute time respiratory syncytial viral bronchiolitis obviously increased and exceeded those of their recovery time and control group.The number of neutrophil in recovery time of respiratory syncytial viral bronchiolitis obviously exceeded those of control group.The level of MMP-9 in all respiratory syncytial viral bronchiolitis cases was significantly higher than those in control group.Conclusion Neutrophil and MMP-9 play an important role in acute bronchiolitis pathogenesis,and are related to the severity of the disease.