帕金森病小鼠前庭神经核内谷氨酸和谷氨酸受体1的表达

Expression of Glutamate and Glutamate Receptor-1 in the Vestibular Nucleus of Mice with Parkinson's Disease

目的研究帕金森病(PD)小鼠前庭神经核内谷氨酸(Glu)及其谷氨酸受体(GluR1)的表达。方法采用免疫细胞化学技术检测帕金森病小鼠前庭神经核内Glu及GluR1的表达,利用透射电镜技术检测前庭神经核内神经元超微结构的改变。结果与对照组相比,在PD小鼠前庭神经核内Glu表达增加(P<0.01),同时GluR1表达减少(P<0.01)。透射电镜的结果显示前庭神经核内神经元超微结构呈病理性改变:神经元线粒体肿胀变性、嵴消失、呈空泡状改变,粗面内质网(RER)扩张,星形胶质细胞呈增生状改变。结论PD小鼠前庭神经核内的Glu及GluR1在神经元内表达的变化及其超微结构的改变,推测其变化与临床运动障碍、平衡失调有一定关系,为帕金森病的临床治疗提供了可靠的形态学依据。

Objective To investigate the expression of glutamate and glutamate receptor-1 in vestibular nucleus of mouse model of Parkinson＇s disease. Methods The distribution of glutamate and glutamate receptor-1 was observed with immunohistochemistry;meanwhile transmission electron microscopy was used to detect ultrastructural changes of neurons in the vestibular nucleus. Results Compared with the control group, Glu expression level was higher in vestibular nucleus but GluR1 expression level was lower in vestibular nucleus in the group of Parkinson＇s disease. What＇s more, transmission electron microscopy showed that the ultrastructure of neurons was partially damaged, which included swelling and partial vacuoles of mitochondria, dilation of granular endoplasmic reticulum, and hyperplasia of astrocytes. Conclusion The expression of Glu and its receptor-1 in the vestibular nucleus of the PD model,which we speculate has a certain relation with clinical dyskinesia and dysbalance, provides a reliable basis for the further study of the morphology of Parkinson＇s disease.