PTEN蛋白在卵巢子宫内膜异位囊肿及卵巢子宫内膜样癌组织中的表达及临床意义

Expression of PTEN protein in endometrial cyst of ovary and ovarian endometrioid carcinoma and its clinical significance

目的探讨卵巢子宫内膜异位囊肿及卵巢子宫内膜样癌组织中抑癌基因PTEN蛋白表达及意义。方法利用免疫组化SP法和蛋白质免疫印迹法检测1985-2005年中国医科大学附属第一医院妇科手术切除的卵巢子宫内膜异位囊肿30例、卵巢子宫内膜样癌标本30例和正常增生期子宫内膜20例组织中PTEN蛋白的表达。结果PTEN蛋白主要定位于细胞浆中,在正常子宫内膜均为阳性表达,在卵巢子宫内膜异位囊肿组织中染色强度和范围较正常子宫内膜弱(0.77±0.08对1.59±0.12,P<0.05);而在卵巢子宫内膜样癌组织中表达明显下降,与正常子宫内膜和卵巢子宫内膜异位囊肿比较,差异有统计学意义(分别为0.55±0.08对1.59±0.12,0.55±0.08对0.77±0.08,P<0.05),且与组织分化程度、临床病理分期及有无子宫内膜异位症病史有关;分期越晚,分化程度越低,蛋白表达越少。结论PTEN蛋白失活可能是卵巢子宫内膜异位囊肿向卵巢子宫内膜样癌发生过程中的重要事件,且检测PTEN蛋白表达对判断患者预后具有一定意义。

Objective To investigate the expression of PTEN protein in endometrial cyst of the ovary and ovarian endometrioid carcinoma as well as to elucidate its significance. Methods Tissues from 30 patients with endometrial cyst of the ovary and 30 patients with ovarian endometrioid carcinoma, and from 20 cases of normal endometrium were analyzed for PTEN gene expression by immunohistochemical technique and Western blot. Results The expression of PTEN protein was mostly located in the cell plasma, which showed high expression in normal endometrium. The expression of PTEN was decreased in endometrial cyst of ovary, compared to that in normal endometrium （P 〈 0. 05 ）, but the expression of PTEN protein decreased significantly in ovarian endometrioid carcinoma, compared to that in the normal endometrium and endometrial cyst of ovary showing significant difference（ P 〈0. 05 ）. The expression of PTEN protein was significantly associated with clinical stage（P 〈 0. 01 ）and differentiation degree of cancer cells（P 〈 0. 05）. In ovarian endometrioid carcinoma PTEN expression was not related to patient＇s age （ P 〉 0. 05 ）. Conclusion It is significant to detect PTEN protein for the clarification of association between endometrial cyst of the ovary and ovarian endometrioid carcinoma,and for the judgment of ovarian endometrioid carcinoma prognosis.