IgY牙膏对远缘链球菌黏附定居的影响

Effect of IgY toothpaste on adhesion and colonization of Streptococcus sobrinus

目的研究IgY牙膏对远缘链球菌体外黏附及体内定居的影响。方法①分别在培养远缘链球菌的培养基中加入浓度分别为2.5mg/ml、1.25mg/ml、0.625mg/ml、0.3125mg/ml的特异性抗变形链球菌IgY抗体和IgY牙膏溶液及对照牙膏(不含IgY抗体,其他成分与IgY牙膏相同)溶液,厌氧培养24h后,用分光光度计测定细菌生长黏附状况。②将24只Wistar大鼠随机分为3组,每组8只,雌雄各半。第1周广谱抑菌,第2周在大鼠牙面上接种S.sobrinus6715,2次/d,50μl/次。从第3周开始按分组用不同的溶液涂抹大鼠牙齿,连续3周。取细菌标本,用MSB和BHI平板培养基定量培养,测定远缘链球菌占总菌落的比例。结果①IgY牙膏组与对照牙膏组比较,黏附率有统计学差异(P<0.05),IgY牙膏与0.3125mg/mlIgY溶液对远缘链球菌的黏附抑制率之间无统计学差异(P>0.05)。②远缘链球菌占总菌落的比例IgY牙膏组与对照牙膏组之间的差异有统计学意义(P<0.05),IgY牙膏组与IgY组之间的差异无统计学意义(P>0.05)。结论IgY牙膏对远缘链球菌的体外黏附和体内定居具有抑制作用。

Objective To investigate in vitro adhesion and in vivo colonization of Streptococcus sobrinus （S. sobrinus ） incubated with IgY toothpaste in rats. Methods ①S. sobrinus were cultured with different concentrations of IgY（2.5 mg/ml, 1.25 mg/ ml,0. 625 mg/ml, 0. 312 5 mg/ml）, IgY toothpaste and control toothpaste, respectively. After culture, the adhesion of S. sobrinus was observed by spectrophotometer. ②Twenty-four Wistar rats were randomly divided into 3 groups. All rats were fed with sterilized water containing Ampicilin 0.25 mg/ml at week 1 and incubated with S. sobrinus6715 twice a day at week 2. At week 3,different solutions were smeared on the surface of the molars for 3 weeks. Bacterium samples were scraped in order to determine the numbers of S. sobrinus by MS plates and general bacterium by BHI plates. The proportion of S. sobrinus to gross colony was measured. Results ①The adhesion rate of S. sobrinus was significantly different between IgY toothpaste group and control toothpaste group（P 〈0.05） ,but not between IgY toothpaste group and 0. 312 5 mg/ml egg yolk IgY group. ②The proportion of S. sobrinus to gross colony had significant difference between IgY toothpaste group and control toothpaste group（P 〈 0.05）, but not between IgY toothpaste group and egg yolk IgY（0. 312 5 mg/ml）group. Conclusion IgY toothpaste can inhibit in vitro adhesion and in vivo colonization of S. sobrinus.