DLC-1基因在原发性肝癌组织中表达的临床意义研究

Clinical Value of the Expression of the DLC-1 in Hepatocellular Carcinoma

目的研究DLC-1基因与肝癌发生、转移乃至复发的临床意义。方法收集南通大学附属肿瘤医院2005年3月-2006年10月间73例HCC及癌旁正常组织（其中12例含癌栓）和24例肝血管瘤手术切除标本，标本均经过病理证实。依据临床以及病理学特征分为高侵袭组币口低侵袭组，采用PCR对所有组织标本DLC-1基因表达进行分析。结果研究发现肝血管瘤、癌旁组织、部分肝癌组织中表达的DLC-1基因大约为450bp，肝癌组织、癌旁组织和肝血管瘤中DLC-1基因表达阳性率依次为69．86％，97．26％和100％，统计学分析这三种组织之间有明显差异（P〈0．05）。DLC-1基因表达在HCC高侵袭组明显低于低侵袭组，二者之间有明显的差异（P〈0．05）。短期随访发现DLC-1表达阴性者的HCC患者中住生存期低于表达阳性患者，且易复发（P〈0.05）。结论定期动态监测高危人群的DLC-1基因表达可能对肝癌患者早期转移复发有一定的临床诊断价值。

Objective To study the clinical value of the expression of the DLC-1 in hepatocellular carcinoma. Methods 73 surgical specimens of human HCC were divided into high invasive and low invasive groups according to their clinicopatho-logical features. 24 cases with non-liver disease as control. The expression of DLC-1 was study in the 73 surgical specimens of human HCC and 24 cases with non-liver disease using PCR. Results A 450-bp fragment of the DLC-1 gene was ampli- fied by PCR. The expression rate of DLC-1 was 69.86% in the HCC,while,that was 97.26% in the corresponding adjacent tissue and it was 100% in corresponding nontumorous livers. There were significantly different among the three tissues（P〈0.05）. The expression of DLC-1 gene in HCC specimens with high invasive group was significantly lower than that with low invasive group（P〈0.05）. The median survival times were significantly different between HCC patients with the ex-pression of the DLC-1 gene and that without which by Short-term follow-up （P〈0.05）. Conclusion Detecting the changes of the expression of DLC-1 in the early stage after hepatectomy of hepatocellular carcinoma may be contributed to the earlier auxiliary diagnosis of the invasiveness,metastasis and recrudescence.