深圳地区男男同性恋人群中HIV-1分子流行病学研究

Study on molecular epidemiology of human immunodeficiency virus type 1 infection in men who have sex with men(MSM) in Shenzhen

目的了解深圳地区男男同性恋人群中艾滋病病毒-Ⅰ型(HIV-1)毒株的分子流行情况,帮助分析HIV-1在该人群中传播的危险因素。方法收集深圳市2006年男男同性恋人群中HIV-1确认为阳性的样本18例,应用套式聚合酶链式反应(Nested-PCR)技术,对该样本膜蛋白基因(env基因)和核心蛋白基因(gag基因)进行扩增,并对各基因区核苷酸序列进行测定和分析。结果18份样本中共存在CRF01 AE、CRF07 BC、CRF08 BC 3种重组毒株及B一种亚型,分别占55.56%(10/18)、16.67%(3/18)、5.56%(1/18)和22.22%(4/18);在env基因区与对应的流行代表株01 AE.TH.90.CM240、07 BC.CN.97.97CN001、08 BC.CN.97.97CNGX 9F、B.US.86.JRFL的基因离散率分别为(8.778±1.296)%、(7.650±0.212)%、11.300%和(13.300±0.819)%;CRF01 AE、CRF07 BC重组株和B亚型组内离散率分别为(10.192±4.179)%、(2.000±0.000)%和(14.367±8.812)%;在gag基因区与对应的流行代表株01 AE.TH.90.CM240、07 BC.CN.97.97CN001、08 BC.CN.97.97CNGX 9F、B.FR.83.HXB2的基因离散率分别为(5.070±1.132)%、(2.167±0.551)%、4.800%和(7.350±1.926)%;CRF01 AE、CRF07 BC重组株和B亚型组内离散率分别为(4.504±1.692)%、(0.700±0.346)%和(10.167±4.352)%。结论深圳地区男男同性恋人群HIV-1流行株以CRF01-AE重组亚型为主,其次是B亚型,也存在CRF07 BC和CRF08BC重组亚型。

Objective To investigate the molecular epidemiology of human immunodeficiency virus-1（HIV-1）infection among men who have sex with men（MSM）in Shenzhen and to study related risk factors so as to predict the epidemic trend and evolution. Method Eighteen HIV-1 positive sera samples of MSM were collected in 2006. HIV-1 env/gag genes were amplified by nested-PCR from RNA.The C2-V3 region of HIV-1 env and P24 region were sequenced for analy- ses. Phylogenetic analyses were performed based on the nucleotide sequence data. Results Among 18 samples, there were 4 HIV-1 strains including CRF01-AE, CRF08-BC, CRF07-BC 3 circulating recombinant forms（CRFs）and B 1 sub- type.Genotyping showed that 55.56% （10/18） were CRF01-AE, 16.67% （3/18） were CRF07-BC, 5.56% （1/18） were CRF08-BC and 22.22% （4/18）were subtype B; In Env region, the AE group showed the highest homology to 01AE. TH. 90. CM240 strain, with the genetic distances of（8.778±1.296）%, and the inner groups distance of（10.192±4.179） %. The 07-BC group showed homology to 07-bc. cn. 97.97cn001 strain, and the genetic distances was（7. 650 ± 0.212） % ,and the inner groups distance was 2.000 % .The 08-BC group showed homology to 08-bc. cn.97.97cngx-9f strain, and the genetic distances was 11. 350 %, The B group showed homology to B. US. 86 .JRF strain, the genetic distances was（13.300±0.819） %, and the inner groups distance was （14.376±8.812 ） % ; In gag region, the AE group showed the highest homology to 01AE.TH. 90. CM240 strain, and to 07-bc. cn. 97.97cn001 strain, and the genetic distances was（2.167±0. 551 ）%, the inner groups distance was（0. 700±0. 346 ）% .The 08-BC group showed the highest homology to 08-be. cn.97.97cngx-9f strain,and the genetic distances was 4.800 % ;The B group showed the highest ho-mology to B. FR. 83. HXB2 strain, the genetic distances was （7.350±1.926 ） %, and the inner groups distance was （10.167±4.352）% .Conclusion CRF01-AE was the main epidemic CRF strains in MSM population in Shenzhen region,followed by Subtype B and CRF07-BC,and CRF08-BC was also prevalent in them.