蛋白酶荧光探针及新型显微成像技术的生物医学应用

Biomedical Applications of Imaging Microscopy Based on Protease-Activated Fluorescent Probe

研究正常生理条件蛋白酶的活化情况仍然存在很多困难,将蛋白酶荧光探针技术和基于各种光学平台的显微成像技术有机地结合起来,可以最大限度地记录活细胞生理条件下蛋白酶活性变化的时空信息。综述了蛋白酶荧光探针技术及其与该类探针应用相关的新型显微成像技术在生物医学光子学领域的应用进展。

It is difficult to determine protease activities under more-or-less physiological conditions, by application of imaging microscopy based on protease-activated fluorescent probe, the spatiotemporal activation of proteases could be monitored in living cells. The imaging microscopy based on protease-activated fluorescent probe in the field of biomedical applications is reviewed.