摘要
目的将含有点突变的白色念珠菌编码基因ERG11克隆至毕氏酵母中进行表达,判断其在白色念珠菌对氟康唑敏感性中的作用。方法将含有点突变的白色念珠菌编码基因ERG11与pPm3.5K载体重组,构建pPiC3.5K/Ca1.6k重组体,经Top10F’鉴定和筛选,电转入毕氏酵母GS115中,经过诱导表达出现目的蛋白后,进行药物敏感性试验,确定点突变与耐药的关系。结果含Y132H突变转化菌株和含Q474K突变的转化菌株对氟康唑的MIC分别上升16倍和8倍,证实其突变与耐药有关。结论氟康唑耐药可发生于白色念珠菌编码基因ERG11的Y132H与Q474K点突变中。
Objective To study the relation between the point mutation of Candida albicans ERGll gene and fluconazole susceptibihty. Method ERGll gene was cloned into pPIC3.5K vector, formed the pPIC3.5K/Ca1.6k, then transformed into Pichia pastoris GSll5 and expressed in various condition. Results The susceptibihty test manifested to Y132H mutation increased by 16 folds MIC and Q474K mutation 8 folds respectively. Condusions Resistance to fluconazole may occur through the Y132H point mutation and Q474K point mutation of Candida albicans gene ERGll respectively.
出处
《国际流行病学传染病学杂志》
CAS
2008年第2期78-81,85,共5页
International Journal of Epidemiology and Infectious Disease
基金
国家自然科学基金(39870011)
关键词
念珠菌
白色
ERG11
毕氏酵母
功能表达
耐药性
Candida albicarts
ERG11
Pichia pastoris
Function expression
Drug resistance