缩宫素诱导的小鼠离体痛经模型的实验方法研究

Study on method of oxytocin induced in vitrodysmenorrhea model in mouse

目的对缩宫素诱发小鼠离体子宫收缩的药理模型的实验条件进行选择和优化。方法使用不同雌激素增加小鼠子宫敏感性，获取小鼠离体子宫，用0．5～10U·L^-1缩宫素诱导离体子宫收缩，观察改变各种处理条件后子宫收缩的变化，对4种离体子宫收缩的描述指标进行比较。结果缩宫素5U·^-1为最适剂量；腹腔注射3d10mg·kg^-1己烯雌酚或苯甲酸雌二醇均可使子宫产生较高的兴奋性；随着体重上升，离体子宫对缩宫素敏感性略有下降，但各组间差异无显著性；给予缩宫素后5～30min内子宫收缩较为恒定；在各项描述指标中平均收缩张力最为灵敏可靠；250μg·L^-1氯丙嗪、1mg·L^-1的维拉帕米及100μg·L^-1硝苯地平可完全抑制离体子宫的收缩。结论缩宫素诱发的离体小鼠子宫收缩模型快速、简便、灵敏，适合用于痛经治疗药物的筛选。

Aim To establish and optimize the dysmenorrhea model of oxytocin induced in vitro uterine contraction in mice. Methods Several kinds of estrogen used to enhance the sensibility of mouse uterus were compared, the uterus was separated from the body, 0. 5 - 10 U · L^-1 oxytocin was used to induce uterine contraction, the variations after changing treatment were observed, and four parameters used to described the uterine contraction were compared Oxytocin in the concentration of 5 U · L ^-1 Results i was the suitable dose; the contractions were constant 5 -30 min after oxytocin was given; the sensibility of uterus showed a little decrease but was not significant when body weight increased; the contractions were stable 5 -30 min after oxytocin was given; the activity of uterus was completely suppressed by 1 mg ~ L-~ verapamil, 100 μg · L^-1 nifedipine and 250 μg · L^-1 chloropromazine. Conclusion Oxytocin induced in vitro mouse uterine contraction model was a convenient, quick and stable in vitro dysmenorrhea model, suitable for screening dysmenorrhea therapy drugs.