二十二碳六烯酸-磷脂脂质体对S_(180)荷瘤小鼠肿瘤抑制和免疫调节作用

Anti-tumor activities and immune regulation of docosahexaenoic acidphosphatidylcholine liposomes in mice bearing S_(180)

目的:探讨二十二碳六烯酸(DHA)-磷脂的抗肿瘤和免疫调节作用。方法:从鸢乌贼卵(Symplectoteuthis oual-aniensis)中提取制备DHA-磷脂,并制备单室脂质体;采用不同剂量的DHA-磷脂脂质体灌胃移植性肿瘤模型小鼠,分别测定其瘤重、脾脏指数、胸腺指数、血清溶血素含量、足跖增厚值和巨噬细胞吞噬能力。结果:DHA-磷脂脂质体能显著抑制小鼠S180肉瘤的生长,其平均抑瘤率为36.87%,提高荷瘤小鼠脾脏脂数(P<0.05),显著提高血清溶血素含量(P<0.05,P<0.01),提高迟发型变态反应水平(P<0.05,P<0.01),促进巨噬细胞吞噬能力(P<0.01)。结论:DHA-磷脂脂质体能全面调节机体的特异性和非特异性免疫功能,抑制肿瘤细胞的生长。

Aim： To investigate the anti-tumor activities and immune regulation of the docosahexaenoic acid （DHA） -phosphatidylcholine （PC） liposomes in S180-bearing mice. Methods： DHA-PC （containing 30% DHA） separated from cuttlefish spawns was used to prepare unilayer liposomes. Different doses of DHA-PC liposomes were given to the transplanted tumor-bearing mice, and the tumor weight, spleen index, thymus index, hemolysin content, delayed type hypersensitivity and the phagocytosis ability of peritoneal macrophage were measured. Hesuits： It was shown that administration of DHA-PC liposomes inhibited the growth of tumor in S180-bearing mice and that the average tumor-inhibitory rate was 36.87%. Furthermore, there were increases of the spleen index （P 〈 0.05）, the hemolysin content （ P 〈 0.05, P 〈 0.01）, delayed allergic level（ P 〈 0.05, P 〈 0.01）, and phagocytosis capability of peritoneal macrophage（ P 〈 0.01）. Conclusion： DHA-PC liposomes could regulate both the spe-cific and non-specific immune functions, and inhibit the growth of tumor cells.