竹叶总黄酮提取纯化工艺研究

Study on Extraction and Purification of Flavonoids from Bamboo Leaves

以芦丁为对照,竹叶总黄酮得率为考察指标,通过正交试验法优选破碎提取竹叶总黄酮的最佳工艺。在此基础上,采用真空薄膜浓缩,超声波脱色,大孔树脂Diaion HP-20吸附分离纯化竹叶总黄酮提取物。结果表明,20g竹叶粗粉最佳提取工艺为用80%(体积分数)乙醇做溶剂,液固比为10∶1(mL∶g)提取1次,5min。最佳分离纯化工艺为:采用大孔树脂Diaion HP-20进行分离富集,以不同浓度的乙醇进行梯度洗脱,洗脱流速为5mL/min,收集并减压浓缩各洗脱部分的总黄酮提取物,精制后的总黄酮纯度可达76%。

Studies on the optimum process of extracting flavonoids from bamboo leaves were carried out by the method of orthogonal test, and flavonoids was determinated using rutin as reference substance. Bamboo leaves extracts were isolated and purificated by vacuum thin film concentration,uhrasonic decolorization, and adsorption with macroreticular resin Diaion HP-20. The result shows that the optimum process of extraction was 80 % （volume fraction） ethanol, 10 times of the weight of raw material, extrac- ting one time for 5 min. The extracts were eluted on Diaion HP-20 resin column with a successive gradient elution system Of H2O- MeOH at 5 mL/min flow rate to obtain extract of flavonoids after vacuum concentration. The purity of flavonoids was 76 %.