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抗原致敏DC诱导CIK细胞对肺腺癌细胞的杀伤作用 被引量：9

The Killing Effect of Antigeu Pulsed Deudritic Cells (DC) Induced CIK Cells on Lung Adenocarcinoma Cells
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摘要 [目的]研究肿瘤抗原致敏的树突状细胞(DC)诱导淋巴因子激活的杀伤细胞(LAK)和细胞因子诱导的杀伤细胞(CIK)对肺腺癌原代细胞的杀伤作用,并与单独LAK、CIK细胞的杀伤效果进行比较。[方法]取健康人外周血单个核细胞(PBMNC),常规诱导出DC、CIK、LAK细胞;用肺癌A549细胞提取的肿瘤抗原冲击DC,倒置显微镜下观察DC形态,流式细胞仪检测DC经抗原冲击和未经抗原冲击后其表型变化;把CIK细胞、DC-CIK细胞、LAK细胞和DC-LAK细胞作为效应细胞,肺腺癌原代细胞作为靶细胞,共分为4组,在10∶1、20∶1、50∶1的效靶比时,进行杀伤试验,使用LDH释放法测定杀伤活性。[结果]DC经肿瘤抗原冲击后在镜下呈典型成熟形态;流式细胞仪检测DC经肿瘤抗原冲击和未经肿瘤抗原冲击其表面分子CD40、CD80、CD86和HLA-DR的表达,前者明显高于后者,两者有显著性差异(P<0.01);DC-CIK细胞对肺腺癌原代细胞的杀伤活性高于DC-LAK细胞、CIK细胞和LAK细胞(P<0.05),随着效靶比的升高,DC-CIK细胞对肺癌细胞的杀伤效应随之增强(P<0.05)。[结论]肿瘤抗原致敏的DC可诱导特异性CIK细胞,DC-CIK细胞对肺腺癌原代细胞的杀伤作用明显高于DC-LAK、CIK、LAK细胞。
作者 李可 吕章春 陈海祥 张立煌
机构地区 永康市第一人民医院 浙江大学医学院免疫研究所
出处 《肿瘤学杂志》 CAS 2008年第4期310-313,共4页 Journal of Chinese Oncology
基金 浙江省科技计划项目(2005C33018)
关键词 树突状细胞 杀伤细胞 肺肿瘤 腺癌
分类号 R734.2 [医药卫生—肿瘤]
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参考文献15

  • 1Schmidt-Wolf IG, Lefterova P, Johnston V, et al. Sensitivity of multidrug resistant tumor cell lines to immunologic effector cells[J]. Cell Immunol, 1996, 169(1 ):85-90.
  • 2Shi Y, Yu J,Cen X, et al. Large-capacity expanded cytokine-induced killer cells and its cytotoxic activity [J]. Sheng Wu Yi Xue Gong Cheng Xue Za Zhi, 2001, 18(1): 94-96.
  • 3Carlens S, Gilljam M, Chambers B J, et al. A new method for in vitro expansion of cytotoxic human an CD3-CD56+ nature killer cells[J]. Hum Immunol, 2001, 62(10):1092- 1098.
  • 4Derek N, Hart J. Dentritic cell: unqiue leukocyte population which control the primary immune response[J]. Blood, 1997, 90(9):3245-3287.
  • 5鄂征.组织培养和分子细胞技术[M].北京:北京出版社,1994.
  • 6杨新静,黄建安,朱一蓓,胡玉敏,闫廷赞,张学光.CIK细胞对肺腺癌细胞株A549体内外杀瘤活性的实验研究[J].苏州大学学报（医学版）,2006,26(4):553-555. 被引量：6
  • 7Maki G, Ex-vivo purging of stem cell autografts using cytotoxic cells[J]. J Hematother Stem Cell Res, 2001, 10(4): 545 -551.
  • 8Carlens S, Gilljam M, Chambers BJ, et al. A new method for in vitro expansion of cytotoxic human CD3 +CD56+ natural killer cells[J]. Hum Immunol, 2001, 62(10):1092- 1098.
  • 9Alvamas JC, Linn YC, Hope EG, et al. Expansion of cytotoxic CD3+CD56+ cells from peripheral blood progenitor cells of patients under going autologous hematopoietic cell transplantation [J]. Biol Blood Marrow Transplant, 2001, 7(4):216-222.
  • 10Hongengs S, Petvises S, Worapongpaiboon S, et al. Generation of CD3+CD56+ cytokine induced killer cells and their in vitro cytotoxicity against pediatric cancer cells [J]. Int J Hematol, 2003, 77(2):175-179.

二级参考文献11

  • 1Schmidt-Wolf IG,Lefterova P,Johnston V,et al.Sensitivity of multidrug-resistant tumor cell lines to immunologic effector cells.Cell Immunol,1996,169:85-90.
  • 2Marten A,Schttker B,Ziske C,et al.Increase of the immunostimulatory effect of dendriticcells by pulsing with CA19-9 protein[J].Immunother,2000,23:464-472.
  • 3Schmidt Wolf IGH,Lefterova P,Johnston V,et al.Sensitivity of multidrug resistant tumor cell lines to immunologic effector cells[J].Cell Immunol,1996,169:85-90.
  • 4Verneris MR,Kornacker M,Mailander V,et al.Resistance of ex vivo expanded CD3^+ CD56+ T cells to Fas mediated apoptosis[J].Cancer Immunol Immunother,2000,49:335-345.
  • 5Marten A,Ziske C,Schttker B,et al.Interactions between dendritic cells and cytokine-induced killer cells lead to an activation of both populations[J].Immunother,2001,24:502-510.
  • 6Schmidt-Wolf Ig,Finke S,Trojaneck B,et al.PhaseI clinical study applying autologous immunological effector cells transfected with the interleukin-2 gene in patients with metastatic renal cancer,colorectal cancer and lymphoma[J].Br J Cancer,1999,81:1009-1016.
  • 7Finke S,Trojaneck B,Lefterova P,et al.Increase of proliferation rate and enhancement of antitumor cytotoxicity of expanded human CD3^+CD56^+ immunologic effector cells by receptor-mediated transfection with the interleukin-7 gene[J].Gene Ther,1998,5:31-39.
  • 8Flieger D,Kufer P,Beier I,et al.A bispecific single-chain antibody directed against EpCAM/CD3 in combination with the cytokines interferon alpha and interleukin-2 efficiently retargets T and CD3^+CD56^+ natural-killer-like T lymphocytes to EpCAM-expressing tumor cells[J].Cancer Immunol Immunother,2000,49:441-448.
  • 9任欢,邢淑贤,徐红薇,宋英晖,商晓舟,周贵生,田景先,李殿俊.CIK的体外增殖及体内外杀瘤活性的实验研究[J].中国肿瘤生物治疗杂志,1999,6(1):17-21. 被引量：66
  • 10古涛,席泓,庄羽美,朱一蓓,邱玉华,戚春建,于葛华,李敏,马泓冰,张学光.分泌独特型免疫球蛋白BALB/c小鼠B细胞淋巴瘤模型的建立[J].中国肿瘤生物治疗杂志,2002,9(3):198-202. 被引量：2

共引文献14

同被引文献142

引证文献9

二级引证文献69

肿瘤学杂志
2008年 第4期

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