牡丹丹皮提取物的纯化研究

Purification of Paeonol from Extracts Cortex Moutan

为了研究牡丹根皮提取物的纯化方法与纯化效果，试验以水蒸气蒸馏法获得的丹皮提取物为样品，用两相溶剂萃取法与酸碱分离法相结合对其纯化重结晶；用薄层层析法与紫外分光光度法和气相色谱质谱法相结合对纯化物进行分析；用气相色谱质谱法测定纯化物的含量。结果表明，两相溶剂萃取法与酸碱分离法相结合使样品中丹皮酚的纯度达到93％，然后用重结晶法，使丹皮酚的纯度由93％提高到98．5％。用薄层层析法进行定性分析，纯化物和标准品的尺厂值相同，说明两者分子量相同。用紫外分光光度法、气相色谱质谱法对纯化物和标准品在波长200-400nm之间扫描，结果在211．80nm和274．20nm处有共同的最大吸收峰，说明两者具有相同组分。用气相色谱质谱法测定祖提物丹皮酚的含量为87．7％、纯化物（Ⅰ）、（Ⅱ）丹皮酚的含量分别为93．0％、98．5％。结果证明此纯化工艺简单，纯化效果好。

Methods of extraction and purification of paeonol from the extracts of cortex moutan were studied. CC-MS was used to measure the content of paeonol in the extract. It was found that with the combination of solvent extraction and acid and base isolation methods, the extracts with a paeonol content of 87. 7% could be purified to 93% ,which would be further purified by recrystalization method with a purity of 98.5%. The purified paeonol had the same Ri value with authentic sample in the thin layer chromatographic test. Paeonol obtained and authentic sample exhibited same UV absorption in 211.80 nm and 274. 20 nm.