HBV对肝癌细胞中mmp-2及nm23表达的影响

Effect of HBV on expressions of mmp-2 and nm23 in HCC cells

目的:研究mmp-2和nm23在无HBV表达的人肝癌细胞株HepG2和持续表达HBV的HepG2细胞株(HepG2.215)中的表达及差异,进一步了解HBV在肝癌侵袭转移中的作用.方法:通过免疫细胞化学、Western Blot检测两组细胞株HepG2和HepG2.215中mmp-2和nm23的表达.结果:肝癌细胞株HepG2.215与HepG2细胞株相比较,其mmp-2表达的水平明显升高(P<0.01),而nm23表达的水平却明显下降(P<0.01).结论:mmp-2和nm23的表达水平能反映肝癌的侵袭转移能力.mmp-2在HepG2.215中的高表达和nm23在HepG2.215的低表达,可提示长时间的HBV感染可能会改变转移相关基因的表达,且有可能会导致肝癌细胞扩散转移.

AIM： To investigate the expression of nm23 and romp-2 in HepG2 cells and HepG2. 215 cells which persistently expresses hepatitis B virus by integrated HBV genome with HepG2, and to further understand the function of HBV in invasion and metastasis of hepatocellular carcinoma （HCC）. METHODS： The expressions of rnmp-2 and nm23 proteins were determined by irnmunecytechemistry and western blot in the HCC cell lines. RESULTS： Compared with HepG2 cells, the expression of rnmp-2 protein increased, and that of nm23 protein decreased in HepG2. 215 cells. CONCLUSION： The expressions of nm23 and romp-2 protein in HCC may reflect the metastasis potential of hepatecellular carcinoma. There is a marked difference in the expression rate of nm23 to rnmp-2 between the HepG2. 215 cells and HepG2 cells. The results indicate a possibility that long time HBV infection could induce the expression profile of metastasis inhibitors, furthermore, cause the invasion and metastasis of hepatocellular carcinoma.