体外细胞基底拉伸加载装置的研制与应用

Development and Application of New Substrate-stretching Device for Cultured Cells

目的:更好地模拟体内细胞所处的力学环境,适应细胞力学信号转导研究的需要。方法:研制了一种适于大面积细胞培养、拉伸,并可以控制时间、应变大小、方向、频率等的单轴双向细胞基底拉伸装置,并利用该装置对细胞基底膜施以拉格朗日应变为10%、频率为1Hz的周期加载。结果:细胞表面形态及内部骨架都发生了变化,细胞长轴取向趋向于与主应变方向垂直,应力纤维排布与细胞取向一致。结论:该装置为研究细胞对机械拉伸的响应提供了多种检测手段。

Objective To simulate the mechanical environment of ceils in vivo and study cellular signal transduction mechanisms. Methods A device was developed which could provide high ceil yield, control the time, strain magnitude, direction and frequency of stretch, and applied 10% cyclic strain to ceil culture substrate with stretch frequency at 1Hz. Results After being stretched, morphology and cytoskeleton of cells were altered. The major axis of ceils and the alignment of stress fibers were vertical to the orientation of cyclic stretch. Conclusion This device provides versatile options for the study on the cellular responses of mechanical loading.[Chinese Medical Equipment Joumal,2008,29（4） ：3-4,8]